OBJECTIVE: To evaluate levels of proinflammatory cytokines and sialidase activity in aerobic vaginitis (AV) in relation to normal vaginal flora and bacterial vaginosis (BV). STUDY DESIGN: In this cross-sectional study, a total of 682 consecutive non-pregnant women attending the gynecology service were assessed and 408 women were included. Vaginal rinsing samples were collected from 223 women with microscopic finding of BV (n=98), aerobic vaginitis (n=25) and normal flora (n=100). Samples were tested for interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, and sialidase activity. RESULTS: Compared to women with normal flora, vaginal levels of IL-1β were highly increased in both BV and AV (p<0.0001). Significantly higher vaginal IL-6 was detected in AV (p<0.0001) but not in BV, in relation to normal flora. Women with AV also presented increased IL-8 levels (p<0.001), while those with BV presented levels similar to normal flora. Sialidase was increased in BV and AV compared with the normal group (p<0.0001) but no difference in sialidase activity was observed between BV and AV. CONCLUSION: A more intense inflammatory host response occurs for AV than for BV when compared with normal flora. Furthermore, the increased sialidase activity in AV and BV indicates that both abnormal vaginal flora types can be harmful to the maintenance of a healthy vaginal environment.
OBJECTIVE: To evaluate levels of proinflammatory cytokines and sialidase activity in aerobic vaginitis (AV) in relation to normal vaginal flora and bacterial vaginosis (BV). STUDY DESIGN: In this cross-sectional study, a total of 682 consecutive non-pregnant women attending the gynecology service were assessed and 408 women were included. Vaginal rinsing samples were collected from 223 women with microscopic finding of BV (n=98), aerobic vaginitis (n=25) and normal flora (n=100). Samples were tested for interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, and sialidase activity. RESULTS: Compared to women with normal flora, vaginal levels of IL-1β were highly increased in both BV and AV (p<0.0001). Significantly higher vaginal IL-6 was detected in AV (p<0.0001) but not in BV, in relation to normal flora. Women with AV also presented increased IL-8 levels (p<0.001), while those with BV presented levels similar to normal flora. Sialidase was increased in BV and AV compared with the normal group (p<0.0001) but no difference in sialidase activity was observed between BV and AV. CONCLUSION: A more intense inflammatory host response occurs for AV than for BV when compared with normal flora. Furthermore, the increased sialidase activity in AV and BV indicates that both abnormal vaginal flora types can be harmful to the maintenance of a healthy vaginal environment.
Authors: Hanneke Borgdorff; Evgeni Tsivtsivadze; Rita Verhelst; Massimo Marzorati; Suzanne Jurriaans; Gilles F Ndayisaba; Frank H Schuren; Janneke H H M van de Wijgert Journal: ISME J Date: 2014-03-06 Impact factor: 10.302
Authors: Elena Shipitsyna; Anna Krysanova; Guzel Khayrullina; Kira Shalepo; Alevtina Savicheva; Alexander Guschin; Magnus Unemo Journal: Mol Diagn Ther Date: 2019-02 Impact factor: 4.074
Authors: Cynthia Rodríguez-Nava; Karen Cortés-Sarabia; Mariana D Avila-Huerta; Edwin J Ortiz-Riaño; Ana K Estrada-Moreno; Luz Del C Alarcón-Romero; Olga Mata-Ruíz; Yolanda Medina-Flores; Amalia Vences-Velázquez; Eden Morales-Narváez Journal: ACS Pharmacol Transl Sci Date: 2021-01-26
Authors: Lorie Benning; Elizabeth T Golub; Kathryn Anastos; Audrey L French; Mardge Cohen; Douglas Gilbert; Patrick Gillevet; Elisaphane Munyazesa; Alan L Landay; Masoumeh Sikaroodi; Gregory T Spear Journal: PLoS One Date: 2014-05-09 Impact factor: 3.240