OBJECTIVE: To describe the relationship between the two mechanisms involved in sIL6R generation in rheumatoid arthritis (RA). METHOD: RA patients were selected from a group of subjects genotyped for the rs8192284 SNP, located at the proteolytic cleavage site of IL-6R. sIL6R and protease levels (ADAM17) were measured and the contribution of alternative splicing in the generation of sIL-6R was evaluated through qRT-PCR. RESULT: Increased sIL-6R plasma levels and expression of spliced isoform generating sIL-6R are genotype dependent. ADAM17 concentrations were independent of the genotype studied. CONCLUSION: Alternative splicing and proteolytic cleavage participate in sIL-6R generation in RA. The rs8192284 polymorphism determines the sIL-6R plasma level through differential proteolytic rupture controlled by ADAM17.
OBJECTIVE: To describe the relationship between the two mechanisms involved in sIL6R generation in rheumatoid arthritis (RA). METHOD:RApatients were selected from a group of subjects genotyped for the rs8192284 SNP, located at the proteolytic cleavage site of IL-6R. sIL6R and protease levels (ADAM17) were measured and the contribution of alternative splicing in the generation of sIL-6R was evaluated through qRT-PCR. RESULT: Increased sIL-6R plasma levels and expression of spliced isoform generating sIL-6R are genotype dependent. ADAM17 concentrations were independent of the genotype studied. CONCLUSION: Alternative splicing and proteolytic cleavage participate in sIL-6R generation in RA. The rs8192284 polymorphism determines the sIL-6R plasma level through differential proteolytic rupture controlled by ADAM17.
Authors: Juan José Nieto-Fontarigo; Francisco Javier Salgado; María Esther San-José; María Jesús Cruz; Luis Valdés; Amparo Pérez-Díaz; Pilar Arias; Montserrat Nogueira; Francisco Javier González-Barcala Journal: Sci Rep Date: 2019-05-17 Impact factor: 4.379
Authors: Alejandro Ibáñez-Costa; Carlos Perez-Sanchez; Alejandra María Patiño-Trives; Maria Luque-Tevar; Pilar Font; Ivan Arias de la Rosa; Cristobal Roman-Rodriguez; Mª Carmen Abalos-Aguilera; Carmen Conde; Antonio Gonzalez; Sergio Pedraza-Arevalo; Mercedes Del Rio-Moreno; Ricardo Blazquez-Encinas; Pedro Segui; Jerusalem Calvo; Rafaela Ortega Castro; Alejandro Escudero-Contreras; Nuria Barbarroja; Mª Angeles Aguirre; Justo P Castaño; Raul M Luque; Eduardo Collantes-Estevez; Chary Lopez-Pedrera Journal: Ann Rheum Dis Date: 2021-10-08 Impact factor: 19.103