AIM: In this study, we investigate whether pH (low) insertion peptide (pHLIP) can target regions of lung injury associated with influenza infection. MATERIALS & METHODS: Fluorophore-conjugated pHLIP was injected intraperitoneally into mice infected with a sublethal dose of H1N1 influenza and visualized histologically. RESULTS: pHLIP specifically targeted inflamed lung tissues of infected mice in the later stages of disease and at sites where alveolar type I and type II cells were depleted. Regions of pHLIP-targeted lung tissue were devoid of peroxiredoxin 6, the lung-abundant antioxidant enzyme, and were deficient in pneumocytes. Interestingly, a pHLIP variant possessing mutations that render it insensitive to pH changes was also able to target damaged lung tissue. CONCLUSION: pHLIP holds potential for delivering therapeutics for lung injury during influenza infection. Furthermore, there may be more than one mechanism that enables pHLIP variants to target inflamed lung tissue.
AIM: In this study, we investigate whether pH (low) insertion peptide (pHLIP) can target regions of lung injury associated with influenza infection. MATERIALS & METHODS: Fluorophore-conjugated pHLIP was injected intraperitoneally into mice infected with a sublethal dose of H1N1influenza and visualized histologically. RESULTS: pHLIP specifically targeted inflamed lung tissues of infected mice in the later stages of disease and at sites where alveolar type I and type II cells were depleted. Regions of pHLIP-targeted lung tissue were devoid of peroxiredoxin 6, the lung-abundant antioxidant enzyme, and were deficient in pneumocytes. Interestingly, a pHLIP variant possessing mutations that render it insensitive to pH changes was also able to target damaged lung tissue. CONCLUSION: pHLIP holds potential for delivering therapeutics for lung injury during influenza infection. Furthermore, there may be more than one mechanism that enables pHLIP variants to target inflamed lung tissue.
Authors: Geng Liu; Sheldon I Feinstein; Yan Wang; Chandra Dodia; Donald Fisher; Kevin Yu; Ye-Shih Ho; Aron B Fisher Journal: Free Radic Biol Med Date: 2010-07-11 Impact factor: 7.376
Authors: Pooja A Kumar; Yuanyu Hu; Yusuke Yamamoto; Neo Boon Hoe; Tay Seok Wei; Dakai Mu; Yan Sun; Lim Siew Joo; Rania Dagher; Elisabeth M Zielonka; De Yun Wang; Bing Lim; Vincent T Chow; Christopher P Crum; Wa Xian; Frank McKeon Journal: Cell Date: 2011-10-28 Impact factor: 41.582
Authors: Yumiko Imai; Keiji Kuba; G Greg Neely; Rubina Yaghubian-Malhami; Thomas Perkmann; Geert van Loo; Maria Ermolaeva; Ruud Veldhuizen; Y H Connie Leung; Hongliang Wang; Haolin Liu; Yang Sun; Manolis Pasparakis; Manfred Kopf; Christin Mech; Sina Bavari; J S Malik Peiris; Arthur S Slutsky; Shizuo Akira; Malin Hultqvist; Rikard Holmdahl; John Nicholls; Chengyu Jiang; Christoph J Binder; Josef M Penninger Journal: Cell Date: 2008-04-18 Impact factor: 41.582
Authors: Sarah A Otieno; Samuel Z Hanz; Bianca Chakravorty; Anqi Zhang; Lukas M Klees; Ming An; Wei Qiang Journal: Proc Natl Acad Sci U S A Date: 2018-11-15 Impact factor: 11.205
Authors: Na Li; Marcus Parrish; Tze Khee Chan; Lu Yin; Prashant Rai; Yamada Yoshiyuki; Nona Abolhassani; Kong Bing Tan; Orsolya Kiraly; Vincent T K Chow; Bevin P Engelward Journal: Cell Mol Life Sci Date: 2015-03-26 Impact factor: 9.261
Authors: Hannah Visca; Michael DuPont; Anna Moshnikova; Troy Crawford; Donald M Engelman; Oleg A Andreev; Yana K Reshetnyak Journal: Mol Imaging Biol Date: 2022-05-23 Impact factor: 3.484
Authors: Linden C Wyatt; Jason S Lewis; Oleg A Andreev; Yana K Reshetnyak; Donald M Engelman Journal: Trends Biotechnol Date: 2017-04-21 Impact factor: 19.536
Authors: Thomas T Tapmeier; Anna Moshnikova; John Beech; Danny Allen; Paul Kinchesh; Sean Smart; Adrian Harris; Alan McIntyre; Donald M Engelman; Oleg A Andreev; Yana K Reshetnyak; Ruth J Muschel Journal: Proc Natl Acad Sci U S A Date: 2015-07-20 Impact factor: 11.205