BACKGROUND: Genomic complexity can predict the clinical course of patients affected by chronic lymphocytic leukemia (CLL) with a normal FISH. However, large studies are still lacking. Here, we analyzed a large series of CLL patients and also carried out the so far largest comparison of FISH versus single-nucleotide polymorphism (SNP) array in this disease. PATIENTS AND METHODS: SNP-array data were derived from a previously reported dataset. RESULTS: Seventy-seven of 329 CLL patients (23%) presented with a normal FISH. At least one large (>5 Mb) genomic aberration was detected by SNP array in 17 of 77 patients (22%); this finding significantly affected TTT. There was no correlation with the presence of TP53 mutations. In multivariate analysis, including age, Binet stage, IGHV genes mutational status and large genomic lesion, the latter three factors emerged as independent prognosticators. The concordance between FISH and SNP array varied between 84 and 97%, depending on the specific genomic locus investigated. CONCLUSIONS: SNP array detected additional large genomic aberrations not covered by the standard FISH panel predicting the outcome of CLL patients.
BACKGROUND: Genomic complexity can predict the clinical course of patients affected by chronic lymphocytic leukemia (CLL) with a normal FISH. However, large studies are still lacking. Here, we analyzed a large series of CLLpatients and also carried out the so far largest comparison of FISH versus single-nucleotide polymorphism (SNP) array in this disease. PATIENTS AND METHODS: SNP-array data were derived from a previously reported dataset. RESULTS: Seventy-seven of 329 CLLpatients (23%) presented with a normal FISH. At least one large (>5 Mb) genomic aberration was detected by SNP array in 17 of 77 patients (22%); this finding significantly affected TTT. There was no correlation with the presence of TP53 mutations. In multivariate analysis, including age, Binet stage, IGHV genes mutational status and large genomic lesion, the latter three factors emerged as independent prognosticators. The concordance between FISH and SNP array varied between 84 and 97%, depending on the specific genomic locus investigated. CONCLUSIONS: SNP array detected additional large genomic aberrations not covered by the standard FISH panel predicting the outcome of CLLpatients.
Authors: Preetesh Jain; Michael Keating; Phillip A Thompson; Long Trinh; Xuemei Wang; William Wierda; Alessandra Ferrajoli; Jan Burger; Hagop Kantarjian; Zeev Estrov; Lynne Abruzzo; Susan O'Brien Journal: Am J Hematol Date: 2015-03-30 Impact factor: 10.047
Authors: Ewelina Grywalska; Jacek Roliński; Marcin Pasiarski; Izabela Korona-Glowniak; Maciej Maj; Agata Surdacka; Agnieszka Grafka; Agnieszka Stelmach-Gołdyś; Michał Zgurski; Stanisław Góźdź; Anna Malm; Piotr Grabarczyk; Elżbieta Starosławska Journal: PLoS One Date: 2015-10-13 Impact factor: 3.240
Authors: Marian Jpl Stevens-Kroef; Eva van den Berg; Daniel Olde Weghuis; Ad Geurts van Kessel; Rolph Pfundt; Matty Linssen-Wiersma; Marloes Benjamins; Trijnie Dijkhuizen; Patricia Jta Groenen; Annet Simons Journal: Mol Cytogenet Date: 2014-01-09 Impact factor: 2.009