Literature DB >> 23363825

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections.

Tine Y Wolff1, Steffen Eickhardt, Maria K Björnsdottir, Claus Moser, Thomas Bjarnsholt, Niels Høiby, Trine R Thomsen.   

Abstract

In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

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Year:  2013        PMID： 23363825      PMCID： PMC3666778          DOI： 10.1128/JCM.00190-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Direct 16S rRNA gene sequencing of polymicrobial culture-negative samples with analysis of mixed chromatograms.

Authors:  Gitte N Hartmeyer; Ulrik S Justesen
Journal:  J Med Microbiol       Date:  2009-12-24       Impact factor: 2.472

2.  Detection of microbial diversity in endocarditis using cultivation-independent molecular techniques.

Authors:  Tine Y Wolff; Claus Moser; Henning Bundgaard; Niels Høiby; Per H Nielsen; Trine R Thomsen
Journal:  Scand J Infect Dis       Date:  2011-08-26

3.  Clinical impact of the use of 16S rRNA sequencing method for the identification of "difficult-to-identify" bacteria in immunocompromised hosts.

Authors:  R Bharadwaj; S Swaminathan; H Salimnia; M Fairfax; A Frey; P H Chandrasekar
Journal:  Transpl Infect Dis       Date:  2011-10-28       Impact factor: 2.228

4.  The bacteriology of chronic venous leg ulcer examined by culture-independent molecular methods.

Authors:  Trine R Thomsen; Martin S Aasholm; Vibeke B Rudkjøbing; Aaron M Saunders; Thomas Bjarnsholt; Michael Givskov; Klaus Kirketerp-Møller; Per H Nielsen
Journal:  Wound Repair Regen       Date:  2010 Jan-Feb       Impact factor: 3.617

5.  Preparation of mycobacterial DNA from blood culture fluids by simple alkali wash and heat lysis method for PCR detection.

Authors:  J K Kulski; T Pryce
Journal:  J Clin Microbiol       Date:  1996-08       Impact factor: 5.948

6.  Culture-negative infective endocarditis caused by Aerococcus urinae.

Authors:  Michal Slany; Tomas Freiberger; Petr Pavlik; Jan Cerny
Journal:  J Heart Valve Dis       Date:  2007-03

7.  Real-time PCR and DNA sequencing for detection and identification of Trichophyton rubrum as a cause of culture negative chronic granulomatous dermatophytosis.

Authors:  Jean-François Durant; Pierre-Alain Fonteyne; Pauline Richez; Liliane Marot; Leila Belkhir; Dominique Tennstedt; Jean-Luc Gala
Journal:  Med Mycol       Date:  2009       Impact factor: 4.076

8.  Direct 16S rRNA gene sequencing from clinical specimens, with special focus on polybacterial samples and interpretation of mixed DNA chromatograms.

Authors:  Oyvind Kommedal; Kristine Kvello; Rune Skjåstad; Nina Langeland; Harald G Wiker
Journal:  J Clin Microbiol       Date:  2009-09-09       Impact factor: 5.948

9.  Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience.

Authors:  Consuelo Ferrer; Jorge L Alió
Journal:  J Ophthalmic Inflamm Infect       Date:  2011-02-23

10.  Use of cultivation-dependent and -independent techniques to assess contamination of central venous catheters: a pilot study.

Authors:  Mette K S Larsen; Trine R Thomsen; Claus Moser; Niels Høiby; Per H Nielsen
Journal:  BMC Clin Pathol       Date:  2008-10-28
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  4 in total

1.  Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples.

Authors:  Camilla Stavnsbjerg; Niels Frimodt-Møller; Claus Moser; Thomas Bjarnsholt
Journal:  BMC Infect Dis       Date:  2017-03-27       Impact factor: 3.090

2.  Increasing Prevalence of Nontuberculous Mycobacteria in Respiratory Specimens from US-Affiliated Pacific Island Jurisdictions1.

Authors:  Chunrong Lin; Chad Russell; Bruce Soll; Dominic Chow; Sapna Bamrah; Richard Brostrom; Wesley Kim; Jerry Scott; Matthew J Bankowski
Journal:  Emerg Infect Dis       Date:  2018-03       Impact factor: 6.883

3.  Phylogenetic analysis of Arthrospira strains from Ordos based on 16S rRNA.

Authors:  Jingnan Zhang; Huiting Xue; Sirui Yan; Haibo Shi; Ling Du; Junfeng Zhang; Da Huo; Ruiping Hu; Hong Yuan; Chunli Ma
Journal:  Sci Rep       Date:  2022-08-23       Impact factor: 4.996

4.  Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures.

Authors:  Nathan Cermak; Manoshi Sen Datta; Arolyn Conwill
Journal:  iScience       Date:  2020-02-14
  4 in total

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