PURPOSE: Synchrotron microbeam radiation therapy (MRT) is a radiosurgery concept in the preclinical stage, developed mainly for brain tumor treatment. Experimental studies suggest that with MRT a better therapeutic index can be obtained than with homogenous field radiotherapy, but the underlying cellular mechanisms need further understanding. The aim of this study was to investigate the dynamics of radiation-induced bystander effects (RIBE) in rats after exposing one brain hemisphere to either MRT or homogenous synchrotron radiation (HSR). MATERIALS AND METHODS: Healthy and tumor-bearing Wistar rats were exposed to doses of 17.5, 35, 70 or 350 Gy, applied either as MRT or HSR to the right cerebral hemisphere. Rats were euthanized at 4, 8 and 12 hours after irradiation to assess the release of bystander signals. Brains and urinary bladders were dissected, and explants for bystander clonogenic reporter assays were set up. RESULTS: Clonogenic survival showed that RIBE occurred in both the non-irradiated brain hemisphere and in bladder of normal and tumor-bearing rats, while the irradiated hemisphere showed the direct effects of radiation. CONCLUSION: The RIBE observed in our reporter cells shows that both MRT and HSR yield a demonstrable abscopal effect after high doses of irradiation; presumably as part of a systemic response.
PURPOSE: Synchrotron microbeam radiation therapy (MRT) is a radiosurgery concept in the preclinical stage, developed mainly for brain tumor treatment. Experimental studies suggest that with MRT a better therapeutic index can be obtained than with homogenous field radiotherapy, but the underlying cellular mechanisms need further understanding. The aim of this study was to investigate the dynamics of radiation-induced bystander effects (RIBE) in rats after exposing one brain hemisphere to either MRT or homogenous synchrotron radiation (HSR). MATERIALS AND METHODS: Healthy and tumor-bearing Wistar rats were exposed to doses of 17.5, 35, 70 or 350 Gy, applied either as MRT or HSR to the right cerebral hemisphere. Rats were euthanized at 4, 8 and 12 hours after irradiation to assess the release of bystander signals. Brains and urinary bladders were dissected, and explants for bystander clonogenic reporter assays were set up. RESULTS: Clonogenic survival showed that RIBE occurred in both the non-irradiated brain hemisphere and in bladder of normal and tumor-bearing rats, while the irradiated hemisphere showed the direct effects of radiation. CONCLUSION: The RIBE observed in our reporter cells shows that both MRT and HSR yield a demonstrable abscopal effect after high doses of irradiation; presumably as part of a systemic response.
Authors: Carmel Mothersill; Cristian Fernandez-Palomo; Jennifer Fazzari; Richard Smith; Elisabeth Schültke; Elke Bräuer-Krisch; Jean Laissue; Christian Schroll; Colin Seymour Journal: Dose Response Date: 2013-08-27 Impact factor: 2.658
Authors: Carl N Sprung; Alesia Ivashkevich; Helen B Forrester; Christophe E Redon; Alexandros Georgakilas; Olga A Martin Journal: Cancer Lett Date: 2013-09-14 Impact factor: 8.679
Authors: Cristian Fernandez-Palomo; Carmel Mothersill; Elke Bräuer-Krisch; Jean Laissue; Colin Seymour; Elisabeth Schültke Journal: PLoS One Date: 2015-03-23 Impact factor: 3.240
Authors: Elisabeth Schültke; Jacques Balosso; Thomas Breslin; Guido Cavaletti; Valentin Djonov; Francois Esteve; Michael Grotzer; Guido Hildebrandt; Alexander Valdman; Jean Laissue Journal: Br J Radiol Date: 2017-07-27 Impact factor: 3.039
Authors: Mohammad Johari Ibahim; Jeffrey C Crosbie; Yuqing Yang; Marina Zaitseva; Andrew W Stevenson; Peter A W Rogers; Premila Paiva Journal: PLoS One Date: 2014-06-19 Impact factor: 3.240
Authors: Richard Smith; Jiaxi Wang; Colin Seymour; Cristian Fernandez-Palomo; Jennifer Fazzari; Elisabeth Schültke; Elke Bräuer-Krisch; Jean Laissue; Christian Schroll; Carmel Mothersill Journal: Dose Response Date: 2018-01-22 Impact factor: 2.658