Pablo Galindo-Moreno1, Pedro Hernández-Cortés2, José Aneiros-Fernández3, Miguel Camara3, Francisco Mesa4, Stephen Wallace5, Francisco O'Valle6. 1. Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, Granada, Spain. 2. Department of Traumatology and Orthopedic Surgery, San Cecilio Clinical Hospital, University of Granada, Granada, Spain. 3. San Cecilio Clinical Hospital, University of Granada University of Granada, Granada, Spain. 4. Periodontology Department, School of Dentistry, University of Granada, Granada, Spain. 5. Department of Periodontology, Columbia University, New York, NY, USA. 6. Pathology Department, School of Medicine, and IBIMER, University of Granada, Granada, Spain.
Abstract
OBJECTIVES: Our objectives were to examine vascular and cellular colonization in anorganic bovine bone (ABB) after 6 months of healing in human maxillary sinus augmentation grafts. MATERIAL AND METHODS: Fifty unilateral maxillary sinus augmentation procedures were performed on 50 consecutive patients. Bone cores were obtained through the implant receptor sites 6 months later and evaluated with morphological image analysis and immunohistochemical techniques. RESULTS: Image analysis revealed a mean of 35.44 ± 16% vital bone, 31.66 ± 15% non-mineralized tissue, and 32.72 ± 25% remnant ABB particles. In our patients, neovascularization in 46.3% of cases was demonstrated within ABB particles 6 months after sinus floor augmentation surgery. Neovascularization of ABB particles was inversely related to age, directly to osteoclast number per mm², and not influenced by habits or disease. CD44-positive cells colonization was found in 74% of cases. An important correlation was found regarding CD44 expression and number of vessel in ABB particles (r = 0.624 P < 0.001, Pearson). Osteopontin expression was detected on the interstitial boundary of bone with ABB particles and within the osteocyte lacunae and bone canaliculi and was relationship with presence of CD44-positive cells inside ABB particles (r = 0.388, P = 0.046, Pearson). CONCLUSION: In conclusion, images compatible with osteone colonized by osteocytes CD44 positive and neovascularization in ABB particles were observed after 6 months of graft maturation. These biologic events have not previously been morphologically and immunohistochemically documented.
OBJECTIVES: Our objectives were to examine vascular and cellular colonization in anorganic bovine bone (ABB) after 6 months of healing in human maxillary sinus augmentation grafts. MATERIAL AND METHODS: Fifty unilateral maxillary sinus augmentation procedures were performed on 50 consecutive patients. Bone cores were obtained through the implant receptor sites 6 months later and evaluated with morphological image analysis and immunohistochemical techniques. RESULTS: Image analysis revealed a mean of 35.44 ± 16% vital bone, 31.66 ± 15% non-mineralized tissue, and 32.72 ± 25% remnant ABB particles. In our patients, neovascularization in 46.3% of cases was demonstrated within ABB particles 6 months after sinus floor augmentation surgery. Neovascularization of ABB particles was inversely related to age, directly to osteoclast number per mm², and not influenced by habits or disease. CD44-positive cells colonization was found in 74% of cases. An important correlation was found regarding CD44 expression and number of vessel in ABB particles (r = 0.624 P < 0.001, Pearson). Osteopontin expression was detected on the interstitial boundary of bone with ABB particles and within the osteocyte lacunae and bone canaliculi and was relationship with presence of CD44-positive cells inside ABB particles (r = 0.388, P = 0.046, Pearson). CONCLUSION: In conclusion, images compatible with osteone colonized by osteocytes CD44 positive and neovascularization in ABB particles were observed after 6 months of graft maturation. These biologic events have not previously been morphologically and immunohistochemically documented.
Authors: Miguel Padial-Molina; Francisco O'Valle; Alejandro Lanis; Francisco Mesa; David M Dohan Ehrenfest; Hom-Lay Wang; Pablo Galindo-Moreno Journal: Biomed Res Int Date: 2015-05-12 Impact factor: 3.411
Authors: Eric Todd Scheyer; Rick Heard; Jim Janakievski; George Mandelaris; Marc L Nevins; Stephen R Pickering; Christopher R Richardson; Bryan Pope; Gregory Toback; Diego Velásquez; Heiner Nagursky Journal: J Clin Periodontol Date: 2016-10-21 Impact factor: 8.728