PURPOSE: The aim of this study was to assess peripheral blood dendritic cell (DC) frequencies and Dendritic Cell-specific intracellular adhesion molecule 3 grabbing non-integrin related (DC-SIGNR) genotyping in healthy individuals, injecting drug users and HIV-1 infected individuals and correlate with different clinical parameters from north India. METHODS: Blood from 30 seronegative healthy individuals, 30 injecting drug users, and 30 patients infected with HIV-1 from North India were collected. Peripheral blood DC frequencies were determined by flow cytometry and repeat region polymorphism in DC-SIGNR was performed by PCR. RESULTS: There was a significantly lower number of DCs and their subsets in patients infected with HIV-1 compared to injecting drug users and healthy individuals. A significant positive correlation of DCs and their subsets with CD4(+) T cells and negative correlation with HIV-1 viral load was found. A salient finding of this study was the association of the heterozygous 7/5 DC-SIGNR genotypes with higher percentage of DCs and their subsets and higher CD4(+) T cell counts and lower viral load compared to the homozygous 7/7 DC-SIGNR genotypes in patients infected with HIV-1. CONCLUSIONS: This is the first study to assess the DC subsets and its association with DC-SIGNR polymorphism in injecting drug users and HIV-1 infected patients and suggests the protective role of 7/5 DC-SIGNR genotypes in HIV-1 infection.
PURPOSE: The aim of this study was to assess peripheral blood dendritic cell (DC) frequencies and Dendritic Cell-specific intracellular adhesion molecule 3 grabbing non-integrin related (DC-SIGNR) genotyping in healthy individuals, injecting drug users and HIV-1 infected individuals and correlate with different clinical parameters from north India. METHODS: Blood from 30 seronegative healthy individuals, 30 injecting drug users, and 30 patients infected with HIV-1 from North India were collected. Peripheral blood DC frequencies were determined by flow cytometry and repeat region polymorphism in DC-SIGNR was performed by PCR. RESULTS: There was a significantly lower number of DCs and their subsets in patients infected with HIV-1 compared to injecting drug users and healthy individuals. A significant positive correlation of DCs and their subsets with CD4(+) T cells and negative correlation with HIV-1 viral load was found. A salient finding of this study was the association of the heterozygous 7/5 DC-SIGNR genotypes with higher percentage of DCs and their subsets and higher CD4(+) T cell counts and lower viral load compared to the homozygous 7/7 DC-SIGNR genotypes in patients infected with HIV-1. CONCLUSIONS: This is the first study to assess the DC subsets and its association with DC-SIGNR polymorphism in injecting drug users and HIV-1 infectedpatients and suggests the protective role of 7/5 DC-SIGNR genotypes in HIV-1 infection.
Authors: Rachel Lubong Sabado; Meagan O'Brien; Abhignya Subedi; Li Qin; Nan Hu; Elizabeth Taylor; Oliver Dibben; Andrea Stacey; Jacques Fellay; Kevin V Shianna; Frederick Siegal; Michael Shodell; Kokila Shah; Marie Larsson; Jeffrey Lifson; Arthur Nadas; Michael Marmor; Richard Hutt; David Margolis; Donald Garmon; Martin Markowitz; Fred Valentine; Persephone Borrow; Nina Bhardwaj Journal: Blood Date: 2010-08-06 Impact factor: 22.113
Authors: Gaby S Steba; Sylvie M Koekkoek; Joost W Vanhommerig; Kees Brinkman; David Kwa; Jan T M Van Der Meer; Maria Prins; Ben Berkhout; Michael Tanck; William A Paxton; Richard Molenkamp; Janke Schinkel Journal: J Infect Dis Date: 2018-01-17 Impact factor: 5.226