Literature DB >> 23349004

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.

Shota Morimoto1, Takenori Tomohiro, Nobuyuki Maruyama, Yasumaru Hatanaka.   

Abstract

A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

Entities:  

Mesh：

Substances：

Year:  2013        PMID： 23349004     DOI： 10.1039/c3cc38594a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  4 in total

1.  Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein.

Authors:  Nobuyuki Maruyama; Tomohiro Goshi; Shigeru Sugiyama; Mayumi Niiyama; Hiroaki Adachi; Kazufumi Takano; Satoshi Murakami; Tsuyoshi Inoue; Yusuke Mori; Hiroyoshi Matsumura; Bunzo Mikami
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-01-28       Impact factor: 1.056

Review 2.  Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs).

Authors:  Dhiraj P Murale; Seong Cheol Hong; Md Mamunul Haque; Jun-Seok Lee
Journal:  Proteome Sci       Date:  2017-06-24       Impact factor: 2.480

Review 3.  Modular Approaches to Synthesize Activity- and Affinity-Based Chemical Probes.

Authors:  Antonie J van der Zouwen; Martin D Witte
Journal:  Front Chem       Date:  2021-04-15       Impact factor: 5.221

4.  A vacuolar sorting receptor-independent sorting mechanism for storage vacuoles in soybean seeds.

Authors:  Nobuyuki Maruyama; Yuki Matsuoka; Kazunori Yokoyama; Kyoko Takagi; Tetsuya Yamada; Hisakazu Hasegawa; Teruhiko Terakawa; Masao Ishimoto
Journal:  Sci Rep       Date:  2018-01-18       Impact factor: 4.379
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.