OBJECTIVE: A hallmark of sepsis and severe systemic inflammatory response syndrome (SIRS) is the massive recruitment of immature neutrophils from the bone marrow into the circulation (left shift, band forms). Their capacity to participate in innate defense against bacteria is ill defined. We aimed at comparing various innate immune functions of mature vs. immature neutrophils circulating during sepsis and SIRS. DESIGN: Prospective, observational cohort study. SETTING: Tertiary level ICU and associated research laboratory. PATIENTS: : Thirty-three ICU patients with sepsis; 12 ICUs with SIRS; 32 healthy volunteers. INTERVENTIONS: Twenty milliliters of whole heparinized blood was used for in vitro studies including neutrophil viability and apoptosis, surface expression of CD16, Toll-like receptors () 4 and TLR2, CD14, MD-2, HLA-DP,-DQ and -DR, and CXCR2, chemotaxis, phagocytosis, bacterial killing, and tumor necrosis factor-α/interleukin-10 baseline intracellular cytokine levels. MEASUREMENTS AND MAIN RESULTS: Immature neutrophils were capable of mediating important innate immune functions such as bacterial phagocytosis and killing via the production of reactive oxygen species, although less efficiently than mature neutrophils. Immature neutrophils had a longer life span and resistance to spontaneous apoptosis, and could mature ex vivo. They expressed lower levels of receptors for bacterial molecules such as CD14 and MD-2 and migrated less efficiently than mature granulocytes. Immature neutrophils had higher basal intracellular tumor necrosis factor-α/interleukin-10 ratio than that of mature neutrophils, suggesting a proinflammatory phenotype. No significant differences were observed between immature neutrophils isolated from patients with sepsis and those from patients with severe SIRS. CONCLUSIONS: Despite their "immaturity", band forms are capable of mediating crucial innate immune functions during severe infections and sepsis. Their fate and capacity to mature in vivo remain to be determined.
OBJECTIVE: A hallmark of sepsis and severe systemic inflammatory response syndrome (SIRS) is the massive recruitment of immature neutrophils from the bone marrow into the circulation (left shift, band forms). Their capacity to participate in innate defense against bacteria is ill defined. We aimed at comparing various innate immune functions of mature vs. immature neutrophils circulating during sepsis and SIRS. DESIGN: Prospective, observational cohort study. SETTING: Tertiary level ICU and associated research laboratory. PATIENTS: : Thirty-three ICU patients with sepsis; 12 ICUs with SIRS; 32 healthy volunteers. INTERVENTIONS: Twenty milliliters of whole heparinized blood was used for in vitro studies including neutrophil viability and apoptosis, surface expression of CD16, Toll-like receptors () 4 and TLR2, CD14, MD-2, HLA-DP,-DQ and -DR, and CXCR2, chemotaxis, phagocytosis, bacterial killing, and tumor necrosis factor-α/interleukin-10 baseline intracellular cytokine levels. MEASUREMENTS AND MAIN RESULTS: Immature neutrophils were capable of mediating important innate immune functions such as bacterial phagocytosis and killing via the production of reactive oxygen species, although less efficiently than mature neutrophils. Immature neutrophils had a longer life span and resistance to spontaneous apoptosis, and could mature ex vivo. They expressed lower levels of receptors for bacterial molecules such as CD14 and MD-2 and migrated less efficiently than mature granulocytes. Immature neutrophils had higher basal intracellular tumor necrosis factor-α/interleukin-10 ratio than that of mature neutrophils, suggesting a proinflammatory phenotype. No significant differences were observed between immature neutrophils isolated from patients with sepsis and those from patients with severe SIRS. CONCLUSIONS: Despite their "immaturity", band forms are capable of mediating crucial innate immune functions during severe infections and sepsis. Their fate and capacity to mature in vivo remain to be determined.
Authors: Min-Young Kwon; Sailaja Ghanta; Julie Ng; Konstantin Tsoyi; James A Lederer; Roderick T Bronson; Souheil El-Chemaly; Su Wol Chung; Xiaoli Liu; Mark A Perrella Journal: Crit Care Med Date: 2020-05 Impact factor: 7.598
Authors: Britta E Jones; Carolina A Herrera; Christian Agosto-Burgos; Joshua Starmer; William A Bass; Caroline J Poulton; Lauren Blazek; Candace D Henderson; Yichun Hu; Susan L Hogan; Peiqi Hu; Hong Xiao; Eveline Y Wu; Dhruti P Chen; J Charles Jennette; Meghan E Free; Ronald J Falk; Dominic J Ciavatta Journal: Kidney Int Date: 2020-05-21 Impact factor: 10.612
Authors: Andriani C Patera; Anne M Drewry; Katherine Chang; Evan R Beiter; Dale Osborne; Richard S Hotchkiss Journal: J Leukoc Biol Date: 2016-09-26 Impact factor: 4.962