Literature DB >> 2334416

Simple and rapid purification of brevin.

H Kurokawa1, W Fujii, K Ohmi, T Sakurai, Y Nonomura.   

Abstract

Brevin or plasma gelsolin, a calcium dependent actin-binding and actin-severing protein, was purified from bovine plasma by a very rapid and simple procedure; ammonium sulfate fractionation and only one step of anion exchange column chromatography by a convenient use. It takes only 24 hrs to complete all the procedure. The purity of brevin prepared by this method was more than 95% on SDS-PAGE and total recovery was much better than previous preparation methods. This brevin preparation has about 8 isomers on 2-D PAGE and strong severing activity on F-actin under electron microscopic observation.

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Year:  1990        PMID： 2334416     DOI： 10.1016/0006-291x(90)92342-w

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  44 in total

1.  Distinct structural changes detected by X-ray fiber diffraction in stabilization of F-actin by lowering pH and increasing ionic strength.

Authors:  T Oda; K Makino; I Yamashita; K Namba; Y Maéda
Journal:  Biophys J       Date:  2001-02       Impact factor: 4.033

2.  Tropomyosin modulates pH dependence of isometric tension.

Authors:  H Fujita; S Ishiwata
Journal:  Biophys J       Date:  1999-09       Impact factor: 4.033

3.  Temperature change does not affect force between single actin filaments and HMM from rabbit muscles.

Authors:  M Kawai; K Kawaguchi; M Saito; S Ishiwata
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

4.  Characterization of single actomyosin rigor bonds: load dependence of lifetime and mechanical properties.

Authors:  T Nishizaka; R Seo; H Tadakuma; K Kinosita; S Ishiwata
Journal:  Biophys J       Date:  2000-08       Impact factor: 4.033

5.  Elementary steps of the cross-bridge cycle in bovine myocardium with and without regulatory proteins.

Authors:  Hideaki Fujita; Daisuke Sasaki; Shin'ichi Ishiwata; Masataka Kawai
Journal:  Biophys J       Date:  2002-02       Impact factor: 4.033

6.  Temperature effect on isometric tension is mediated by regulatory proteins tropomyosin and troponin in bovine myocardium.

Authors:  Hideaki Fujita; Masataka Kawai
Journal:  J Physiol       Date:  2002-02-15       Impact factor: 5.182

7.  Purification of native myosin filaments from muscle.

Authors:  C Hidalgo; R Padrón; R Horowitz; F Q Zhao; R Craig
Journal:  Biophys J       Date:  2001-11       Impact factor: 4.033

8.  VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations.

Authors:  E L Bearer; J M Prakash; R D Manchester; P G Allen
Journal:  Cell Motil Cytoskeleton       Date:  2000-12

9.  The effect of tropomyosin on force and elementary steps of the cross-bridge cycle in reconstituted bovine myocardium.

Authors:  Hideaki Fujita; Xiaoying Lu; Madoka Suzuki; Shin'ichi Ishiwata; Masataka Kawai
Journal:  J Physiol       Date:  2004-01-23       Impact factor: 5.182

10.  Effects of tropomyosin internal deletion Delta23Tm on isometric tension and the cross-bridge kinetics in bovine myocardium.

Authors:  Xiaoying Lu; Larry S Tobacman; Masataka Kawai
Journal:  J Physiol       Date:  2003-09-18       Impact factor: 5.182
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