BACKGROUND: The purpose of this work was to engineer polymeric nanoparticles to encapsulate and deliver 2-methoxyestradiol, a potential antitumor drug for treatment of uterine leiomyoma (fibroids), the most common hormone-dependent pathology affecting women of reproductive age. METHODS/ RESULTS: Encapsulation efficiency and drug release from the nanoparticles were monitored by HPLC. Cell morphology and in vitro cytotoxicity experiments were carried out in a human leiomyoma cell line. The nanoparticles displayed high encapsulation efficiency (>86%), which was verified by differential scanning calorimetry and x-ray diffraction. Excellent long-term stability of the nanoparticles and gradual drug release without burst were also observed. Cellular uptake of fluorescent nanoparticles was confirmed by confocal imaging. The drug-loaded poly(lactic acid) and poly(lactic-co-glycolic acid) nanoparticles induced cytotoxicity in human leiomyoma cells to a significantly greater extent than the free drug at 0.35 µM. CONCLUSION: This novel approach represents a potential fertility-preserving alternative to hysterectomy.
BACKGROUND: The purpose of this work was to engineer polymeric nanoparticles to encapsulate and deliver 2-methoxyestradiol, a potential antitumor drug for treatment of uterine leiomyoma (fibroids), the most common hormone-dependent pathology affecting women of reproductive age. METHODS/ RESULTS: Encapsulation efficiency and drug release from the nanoparticles were monitored by HPLC. Cell morphology and in vitro cytotoxicity experiments were carried out in a humanleiomyoma cell line. The nanoparticles displayed high encapsulation efficiency (>86%), which was verified by differential scanning calorimetry and x-ray diffraction. Excellent long-term stability of the nanoparticles and gradual drug release without burst were also observed. Cellular uptake of fluorescent nanoparticles was confirmed by confocal imaging. The drug-loaded poly(lactic acid) and poly(lactic-co-glycolic acid) nanoparticles induced cytotoxicity in humanleiomyoma cells to a significantly greater extent than the free drug at 0.35 µM. CONCLUSION: This novel approach represents a potential fertility-preserving alternative to hysterectomy.
Authors: William L Dahut; Nehal J Lakhani; James L Gulley; Philip M Arlen; Elise C Kohn; Herbert Kotz; Debbie McNally; Allyson Parr; Diana Nguyen; Sherry X Yang; Seth M Steinberg; Jürgen Venitz; Alex Sparreboom; William D Figg Journal: Cancer Biol Ther Date: 2006-01-22 Impact factor: 4.742
Authors: Justin L Ricker; Zhong Chen; Xin Ping Yang; Victor S Pribluda; Glenn M Swartz; Carter Van Waes Journal: Clin Cancer Res Date: 2004-12-15 Impact factor: 12.531
Authors: M F Zambaux; F Bonneaux; R Gref; P Maincent; E Dellacherie; M J Alonso; P Labrude; C Vigneron Journal: J Control Release Date: 1998-01-02 Impact factor: 9.776
Authors: Phoebe A Stapleton; Cody E Nichols; Jinghai Yi; Carroll R McBride; Valerie C Minarchick; Danielle L Shepherd; John M Hollander; Timothy R Nurkiewicz Journal: Nanotoxicology Date: 2015-09-04 Impact factor: 5.913
Authors: Mostafa A Borahay; Xiao Fang; Jacques G Baillargeon; Gokhan S Kilic; Darren F Boehning; Yong-Fang Kuo Journal: Am J Obstet Gynecol Date: 2016-06-28 Impact factor: 8.661