Literature DB >> 23340874

Fluorogenic label to quantify the cytosolic delivery of macromolecules.

Tzu-Yuan Chao1, Ronald T Raines.   

Abstract

The delivery of a macromolecule to the cytosol of human cells is assessed by using a pendant di-O-glycosylated derivative of fluorescein. Its fluorescence is unmasked by Escherichia coliβ-galactosidase installed in the cytosol. Background is diminished by using RNAi to suppress the expression of GLB1, which encodes a lysosomal β-galactosidase. This strategy was used to quantify the cytosolic entry of a highly cationic protein, ribonuclease A.

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Year:  2013        PMID： 23340874      PMCID： PMC3588161          DOI： 10.1039/c3mb25552b

Source DB:  PubMed          Journal:  Mol Biosyst        ISSN： 1742-2051


  36 in total

1.  Ribonuclease A.

Authors:  Ronald T. Raines
Journal:  Chem Rev       Date:  1998-05-07       Impact factor: 60.622

2.  Selective esterase-ester pair for targeting small molecules with cellular specificity.

Authors:  Lin Tian; Yunlei Yang; Laura M Wysocki; Alma C Arnold; Amy Hu; Balaji Ravichandran; Scott M Sternson; Loren L Looger; Luke D Lavis
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-12       Impact factor: 11.205

Review 3.  Natural and engineered ribonucleases as potential cancer therapeutics.

Authors:  Ulrich Arnold; Renate Ulbrich-Hofmann
Journal:  Biotechnol Lett       Date:  2006-08-11       Impact factor: 2.461

4.  Intracellular pathway of Onconase that enables its delivery to the cytosol.

Authors:  Montserrat Rodríguez; Gerard Torrent; Montserrat Bosch; Fabienne Rayne; Jean-François Dubremetz; Marc Ribó; Antoni Benito; Maria Vilanova; Bruno Beaumelle
Journal:  J Cell Sci       Date:  2007-03-20       Impact factor: 5.285

Review 5.  How to address CPP and AMP translocation? Methods to detect and quantify peptide internalization in vitro and in vivo (Review).

Authors:  Sónia Troeira Henriques; Manuel Nuno Melo; Miguel A R B Castanho
Journal:  Mol Membr Biol       Date:  2007 May-Jun       Impact factor: 2.857

Review 6.  The design of guanidinium-rich transporters and their internalization mechanisms.

Authors:  Paul A Wender; Wesley C Galliher; Elena A Goun; Lisa R Jones; Thomas H Pillow
Journal:  Adv Drug Deliv Rev       Date:  2007-11-09       Impact factor: 15.470

Review 7.  Macromolecular cytosolic delivery: cell membranes as the primary obstacle.

Authors:  G M Larson; K D Lee
Journal:  Arch Pharm Res       Date:  1998-12       Impact factor: 4.946

8.  Bafilomycin inhibits proton flow through the H+ channel of vacuolar proton pumps.

Authors:  B P Crider; X S Xie; D K Stone
Journal:  J Biol Chem       Date:  1994-07-01       Impact factor: 5.157

9.  Ribonuclease A variants with potent cytotoxic activity.

Authors:  P A Leland; L W Schultz; B M Kim; R T Raines
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

10.  Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.

Authors:  A Plovins; A M Alvarez; M Ibañez; M Molina; C Nombela
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792
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  10 in total

Review 1.  Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging.

Authors:  Wen Chyan; Ronald T Raines
Journal:  ACS Chem Biol       Date:  2018-07-06       Impact factor: 5.100

2.  Esterification Delivers a Functional Enzyme into a Human Cell.

Authors:  Valerie T Ressler; Kalie A Mix; Ronald T Raines
Journal:  ACS Chem Biol       Date:  2019-03-11       Impact factor: 5.100

3.  Understanding Cell Penetration of Cyclic Peptides.

Authors:  Patrick G Dougherty; Ashweta Sahni; Dehua Pei
Journal:  Chem Rev       Date:  2019-05-14       Impact factor: 60.622

4.  Knockout of the Ribonuclease Inhibitor Gene Leaves Human Cells Vulnerable to Secretory Ribonucleases.

Authors:  Sydney P Thomas; Eunji Kim; Jin-Soo Kim; Ronald T Raines
Journal:  Biochemistry       Date:  2016-11-08       Impact factor: 3.162

5.  Fluorescence correlation spectroscopy reveals highly efficient cytosolic delivery of certain penta-arg proteins and stapled peptides.

Authors:  Jonathan R LaRochelle; Garrett B Cobb; Angela Steinauer; Elizabeth Rhoades; Alanna Schepartz
Journal:  J Am Chem Soc       Date:  2015-02-13       Impact factor: 15.419

6.  Contribution of electrostatics to the binding of pancreatic-type ribonucleases to membranes.

Authors:  Nadia K Sundlass; Chelcie H Eller; Qiang Cui; Ronald T Raines
Journal:  Biochemistry       Date:  2013-08-30       Impact factor: 3.162

7.  Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus.

Authors:  Hsin-Yi Chiu; Jack A Bates; Jonas Helma; Hanna Engelke; Hartmann Harz; Thomas Bein; Heinrich Leonhardt
Journal:  Nucleus       Date:  2018       Impact factor: 4.197

8.  Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins.

Authors:  Rebecca F Wissner; Angela Steinauer; Susan L Knox; Alexander D Thompson; Alanna Schepartz
Journal:  ACS Cent Sci       Date:  2018-09-27       Impact factor: 14.553

9.  Selective Cleavage at CCA Ends and Anticodon Loops of tRNAs by Stress-Induced RNases.

Authors:  Yasutoshi Akiyama; Shawn M Lyons; Marta M Fay; Yoshihisa Tomioka; Takaaki Abe; Paul J Anderson; Pavel Ivanov
Journal:  Front Mol Biosci       Date:  2022-03-01

10.  Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor.

Authors:  Sydney P Thomas; Trish T Hoang; Valerie T Ressler; Ronald T Raines
Journal:  RNA       Date:  2018-05-10       Impact factor: 4.942
  10 in total

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