| Literature DB >> 23333648 |
Eduardo Gonçalves Paterson Fox1, Daniel Russ Solis, Lucilene Delazari Dos Santos, Jose Roberto Aparecido Dos Santos Pinto, Anally Ribeiro da Silva Menegasso, Rafael Cardoso Maciel Costa Silva, Mario Sergio Palma, Odair Correa Bueno, Ednildo de Alcântara Machado.
Abstract
The invasive fire ant Solenopsis invicta is medically important because its venom is highly potent. However, almost nothing is known about fire ant venom proteins because obtaining even milligram-amounts of these proteins has been prohibitively challenging. We present a simple and fast method of obtaining whole venom compounds from large quantities of fire ants. For this, we separate the ants are from the nest soil, immerse them in dual-phase mixture of apolar organic solvent and water, and evaporate each solvent phase in separate. The remaining extract from the aqueous phase is largely made up of ant venom proteins. We confirmed this by using 2D gel electrophoresis while also demonstrating that our new approach yields the same proteins obtained by other authors using less efficient traditional methods.Entities:
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Year: 2013 PMID： 23333648 DOI： 10.1016/j.toxicon.2012.12.009
Source DB: PubMed Journal: Toxicon ISSN： 0041-0101 Impact factor: 3.033