Sumei Sha1, Yong Gu, Bin Xu, Hao Hu, Yan Yang, Xiangyun Kong, Kaichun Wu. 1. State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi Province, PR China. shasumei001@163.com
Abstract
BACKGROUND: Studies have demonstrated the implication of HOXB9 in tumorigenesis, but its role in gastric carcinoma remains unknown. AIMS: To investigate the expression and prognostic value of HOXB9 in patients with gastric carcinoma. METHODS: The localization and expression of HOXB9 in gastric cancer cells lines were detected by immunofluorescence and western blot. The mRNA and protein expression level of HOXB9 was detected in subjects with gastric carcinoma and paired non-cancerous tissues. Correlation between HOXB9 expression and clinicopathological parameters, the association of HOXB9 expression with the patients' survival rate was also assessed. RESULTS: HOXB9 was predominantly localized in the cell nucleus. A significant decrease in HOXB9 intensity in poorly differentiated gastric cancer cells is evident (P<0.01). A lower mRNA and protein expression level of HOXB9 was detected in gastric carcinoma (P<0.01). Decreased expression of HOXB9, poorly differentiation status and the presence of lymph node metastasis predict shorter overall survival (P<0.05). Patients without HOXB9 expression had a lower overall survival rate (P<0.01). Multivariate Cox regression analysis showed HOXB9 was an independent prognostic factor in gastric carcinoma (P<0.01). CONCLUSIONS: HOXB9 is down-regulation in gastric carcinoma and may be a novel prognostic marker for poorer clinical outcome for patients with gastric carcinoma.
BACKGROUND: Studies have demonstrated the implication of HOXB9 in tumorigenesis, but its role in gastric carcinoma remains unknown. AIMS: To investigate the expression and prognostic value of HOXB9 in patients with gastric carcinoma. METHODS: The localization and expression of HOXB9 in gastric cancer cells lines were detected by immunofluorescence and western blot. The mRNA and protein expression level of HOXB9 was detected in subjects with gastric carcinoma and paired non-cancerous tissues. Correlation between HOXB9 expression and clinicopathological parameters, the association of HOXB9 expression with the patients' survival rate was also assessed. RESULTS:HOXB9 was predominantly localized in the cell nucleus. A significant decrease in HOXB9 intensity in poorly differentiated gastric cancer cells is evident (P<0.01). A lower mRNA and protein expression level of HOXB9 was detected in gastric carcinoma (P<0.01). Decreased expression of HOXB9, poorly differentiation status and the presence of lymph node metastasis predict shorter overall survival (P<0.05). Patients without HOXB9 expression had a lower overall survival rate (P<0.01). Multivariate Cox regression analysis showed HOXB9 was an independent prognostic factor in gastric carcinoma (P<0.01). CONCLUSIONS:HOXB9 is down-regulation in gastric carcinoma and may be a novel prognostic marker for poorer clinical outcome for patients with gastric carcinoma.
Authors: Lav Darda; Fahad Hakami; Richard Morgan; Craig Murdoch; Daniel W Lambert; Keith D Hunter Journal: PLoS One Date: 2015-04-10 Impact factor: 3.240
Authors: Joao Paulo Oliveira-Costa; Alex Fiorini de Carvalho; da Giorgia Gobbi da Silveira; Peter Amaya; Yongqi Wu; Kyoung-Joo Jenny Park; Mabel Pinilla Gigliola; Maryam Lustberg; Marcilei Eliza Cavicchioli Buim; Elisa Napolitano Ferreira; Luiz Paulo Kowalski; Jeffrey J Chalmers; Fernando Augusto Soares; Dirce Maria Carraro; Alfredo Ribeiro-Silva Journal: Oncotarget Date: 2015-08-28