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Literature DB >> 23329513

Production of primary human CD4⁺ T cell lines and clones.

Abstract

Tetramer staining of CD4(+) T cells is a valuable technique in immunology for detecting rare autoreactive T cells. Generating clones or cell lines from autoantigen tetramer-positive CD4(+) T cells allows further characterization and phenotyping of autoreactive cells.

Entities: Chemical Disease Gene Species

Mesh：

Year: 2013 PMID： 23329513 PMCID： PMC4018300 DOI： 10.1007/978-1-62703-218-6_40

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

15 in total

1. Distinct T cell interactions with HLA class II tetramers characterize a spectrum of TCR affinities in the human antigen-specific T cell response.

Authors: S Reichstetter; R A Ettinger; A W Liu; J A Gebe; G T Nepom; W W Kwok
Journal: J Immunol Date: 2000-12-15 Impact factor: 5.422

2. GAD65-specific CD4+ T-cells with high antigen avidity are prevalent in peripheral blood of patients with type 1 diabetes.

Authors: Helena Reijonen; Roberto Mallone; Anne-Kristin Heninger; Elsa M Laughlin; Sharon A Kochik; Ben Falk; William W Kwok; Carla Greenbaum; Gerald T Nepom
Journal: Diabetes Date: 2004-08 Impact factor: 9.461

3. Functional avidity directs T-cell fate in autoreactive CD4+ T cells.

Authors: Roberto Mallone; Sharon A Kochik; Helena Reijonen; Bryan Carson; Steven F Ziegler; William W Kwok; Gerald T Nepom
Journal: Blood Date: 2005-07-19 Impact factor: 22.113

4. Ultra-sensitive detection of rare T cell clones.

Authors: Harlan Robins; Cindy Desmarais; Jessica Matthis; Robert Livingston; Jessica Andriesen; Helena Reijonen; Christopher Carlson; Gerold Nepom; Cassian Yee; Karen Cerosaletti
Journal: J Immunol Methods Date: 2011-09-10 Impact factor: 2.303

5. Detection of GAD65-specific T-cells by major histocompatibility complex class II tetramers in type 1 diabetic patients and at-risk subjects.

Authors: Helena Reijonen; Erik J Novak; Sharon Kochik; Anne Heninger; Andrew W Liu; William W Kwok; Gerald T Nepom
Journal: Diabetes Date: 2002-05 Impact factor: 9.461

Production of primary human CD4⁺ T cell lines and clones.

1. Distinct T cell interactions with HLA class II tetramers characterize a spectrum of TCR affinities in the human antigen-specific T cell response.

2. GAD65-specific CD4+ T-cells with high antigen avidity are prevalent in peripheral blood of patients with type 1 diabetes.

3. Functional avidity directs T-cell fate in autoreactive CD4+ T cells.

4. Ultra-sensitive detection of rare T cell clones.

5. Detection of GAD65-specific T-cells by major histocompatibility complex class II tetramers in type 1 diabetic patients and at-risk subjects.

Review 6. Defining antigen-specific responses with human MHC class II tetramers.

Review 7. Use of HLA class II tetramers in tracking antigen-specific T cells and mapping T-cell epitopes.

8. Detection of CD4+ autoreactive T cells in T1D using HLA class II tetramers.

9. Islet-specific glucose-6-phosphatase catalytic subunit-related protein-reactive CD4+ T cells in human subjects.

10. Differential recognition and activation thresholds in human autoreactive GAD-specific T-cells.