Literature DB >> 23329498

Preparation of dendritic cells by in vitro cultures.

Mirjana Weimershaus1, Peter van Endert2.   

Abstract

In vitro cultures of bone marrow-derived precursors are a convenient method for generating dendritic cells (DC). This method additionally overcomes the problem of low availability of certain DC types, DC heterogeneity, and laborious procedures encountered using ex vivo isolation protocols.Here we describe two standard protocols for in vitro differentiation of steady-state DC equivalents with Fms-like tyrosine kinase 3 ligand (Flt3L) and inflammatory-like DC using granulocyte-macrophages-colony-stimulating factor (GM-CSF). These protocols allow for obtaining up to 2 × 10(8) CD11c(high) inflammatory-like DC and up to 5 × 10(6) equivalents of each CD8+ and CD8- conventional DC and plasmacytoid DC.

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Year:  2013        PMID： 23329498     DOI： 10.1007/978-1-62703-218-6_25

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Screening Identifies Thimerosal as a Selective Inhibitor of Endoplasmic Reticulum Aminopeptidase 1.

Authors:  Athanasios Stamogiannos; Athanasios Papakyriakou; Francois-Xavier Mauvais; Peter van Endert; Efstratios Stratikos
Journal:  ACS Med Chem Lett       Date:  2016-05-31       Impact factor: 4.345

2.  Robust expansion of dendritic cells in vivo by hydrodynamic FLT3L-FC gene transfer.

Authors:  Hua Tu; Thomas M Burke; Cecilia Oderup; Kexin Huang; Kathryn Wong; Susanna Lewén; Melissa LaJevic; Brian A Zabel
Journal:  J Immunol Methods       Date:  2014-07-24       Impact factor: 2.303

3.  Differentially Tolerized Mouse Antigen Presenting Cells Share a Common miRNA Signature Including Enhanced mmu-miR-223-3p Expression Which Is Sufficient to Imprint a Protolerogenic State.

Authors:  Matthias Bros; Mahmoud Youns; Verena Kollek; Diana Buchmüller; Franziska Bollmann; Ean-Jeong Seo; Jonathan Schupp; Evelyn Montermann; Svetlana Usanova; Hartmut Kleinert; Thomas Efferth; Angelika B Reske-Kunz
Journal:  Front Pharmacol       Date:  2018-08-17       Impact factor: 5.810

4.  CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.

Authors:  Dimitar B Iliev; Leidy Lagos; Hanna L Thim; Sven M Jørgensen; Aleksei Krasnov; Jorunn B Jørgensen
Journal:  Front Immunol       Date:  2019-03-13       Impact factor: 7.561

5.  No major role for insulin-degrading enzyme in antigen presentation by MHC molecules.

Authors:  Slobodan Culina; François-Xavier Mauvais; Hsiang-Ting Hsu; Anne Burgevin; Suzanne Guénette; Anna Moser; Peter van Endert
Journal:  PLoS One       Date:  2014-02-07       Impact factor: 3.240
  5 in total

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