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Literature DB >> 23329132

Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.

Yadveer S Grewal¹, Muhammad J A Shiddiky, Sean A Gray, Kris M Weigel, Gerard A Cangelosi, Matt Trau.

Abstract

Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 2013 PMID： 23329132 PMCID： PMC3564640 DOI： 10.1039/c2cc38882k

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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