AIM: To investigate the relationship between c.343A>G and c.2216A>C polymorphism sites in the CDH17 gene and colorectal carcinoma. METHODS: Ninety-three non-consanguineous colorectal carcinoma patients admitted to the Department of Oncology at the First Affiliated Hospital of Zhengzhou University were included in this study. Ninety-three peripheral venous blood samples, of approximately one milliliter from each patient, were collected between December 2009 and August 2010. The genomic DNA of these peripheral venous blood samples were extracted and purified using a Fermentas Genomic DNA Purification Kit (Fermentas, CA) according to the manufacturer's protocol. The single nucleotide polymorphisms (SNPs) of the liver-intestine cadherin (CDH17) gene c.343A>G and c.2216A>C were determined by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP) in 93 peripheral venous blood samples from patients suffering with colorectal carcinoma. Typical samples that showed different migration bands in SSCP were confirmed by sequencing. Directed DNA sequencing was used to check the correctness of the genotype results from the PCR-SSCP method. RESULTS: There was a significant association between the c.2216 A>C SNPs of the CDH17 gene and the tumor-node-metastasis (TNM) grade, as well as with lymph node status, in 93 peripheral venous blood samples from colorectal carcinoma patients. The genotype frequencies of A/C, A/A, and C/C were 12.90%, 33.33% and 53.76%, respectively. There was a significant correlation between lymph node metastasis, TNM grade, and the genotype distribution (P < 0.05). The C/C genotype raised the risk of lymph node metastasis and the TNM grade. There was a significant difference in the TNM grade and lymph node metastasis between the A/A and C/C genotypes (P = 0.003 and P = 0.013, respectively). Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis and have a higher TNM grade. The difference between the TNM grades, as well as the lymph node metastasis of the two alleles, was statistically significant (P < 0.01). CONCLUSION: The SNPs of the CDH17 gene c.2216 A>C might be clinically important in the prognosis of colorectal carcinoma.
AIM: To investigate the relationship between c.343A>G and c.2216A>C polymorphism sites in the CDH17 gene and colorectal carcinoma. METHODS: Ninety-three non-consanguineous colorectal carcinomapatients admitted to the Department of Oncology at the First Affiliated Hospital of Zhengzhou University were included in this study. Ninety-three peripheral venous blood samples, of approximately one milliliter from each patient, were collected between December 2009 and August 2010. The genomic DNA of these peripheral venous blood samples were extracted and purified using a Fermentas Genomic DNA Purification Kit (Fermentas, CA) according to the manufacturer's protocol. The single nucleotide polymorphisms (SNPs) of the liver-intestine cadherin (CDH17) gene c.343A>G and c.2216A>C were determined by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP) in 93 peripheral venous blood samples from patients suffering with colorectal carcinoma. Typical samples that showed different migration bands in SSCP were confirmed by sequencing. Directed DNA sequencing was used to check the correctness of the genotype results from the PCR-SSCP method. RESULTS: There was a significant association between the c.2216 A>C SNPs of the CDH17 gene and the tumor-node-metastasis (TNM) grade, as well as with lymph node status, in 93 peripheral venous blood samples from colorectal carcinomapatients. The genotype frequencies of A/C, A/A, and C/C were 12.90%, 33.33% and 53.76%, respectively. There was a significant correlation between lymph node metastasis, TNM grade, and the genotype distribution (P < 0.05). The C/C genotype raised the risk of lymph node metastasis and the TNM grade. There was a significant difference in the TNM grade and lymph node metastasis between the A/A and C/C genotypes (P = 0.003 and P = 0.013, respectively). Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis and have a higher TNM grade. The difference between the TNM grades, as well as the lymph node metastasis of the two alleles, was statistically significant (P < 0.01). CONCLUSION: The SNPs of the CDH17 gene c.2216 A>C might be clinically important in the prognosis of colorectal carcinoma.
Authors: Xiao Qi Wang; John M Luk; Mercè Garcia-Barcelo; Xiaoping Miao; Pauline P Leung; David W Ho; Siu Tim Cheung; Brian Y Lam; Cindy K Cheung; Ashley S Wong; Silvana S Lau; Man Ting So; Wan Ching Yu; Qi Cai; Karen S Liu; Chee Kin Hui; George K Lau; Ronnie T P Poon; John Wong; Sheung Tat Fan Journal: Clin Cancer Res Date: 2006-09-01 Impact factor: 12.531
Authors: Xiao Qi Wang; John M Luk; Pauline P Leung; Bonnie W Wong; Eric J Stanbridge; Sheung Tat Fan Journal: Clin Cancer Res Date: 2005-01-15 Impact factor: 12.531
Authors: E Papadopoulou; K Simopoulos; G Tripsianis; I Tentes; K Anagnostopoulos; E Sivridis; G Galazios; A Kortsaris Journal: Neoplasma Date: 2007 Impact factor: 2.575
Authors: B Kreft; D Berndorff; A Böttinger; S Finnemann; D Wedlich; M Hortsch; R Tauber; R Gessner Journal: J Cell Biol Date: 1997-03-10 Impact factor: 10.539
Authors: Gururaj Shivange; Karol Urbanek; Piotr Przanowski; Justin S A Perry; James Jones; Robert Haggart; Christina Kostka; Tejal Patki; Edward Stelow; Yuliya Petrova; Danielle Llaneza; Marty Mayo; Kodi S Ravichandran; Charles N Landen; Sanchita Bhatnagar; Jogender Tushir-Singh Journal: Cancer Cell Date: 2018-08-13 Impact factor: 31.743