| Literature DB >> 23325663 |
Johann W Bauer1, Eva M Murauer, Verena Wally, Ulrich Koller.
Abstract
Spliceosome-mediated RNA trans-splicing (SMaRT) is a tool that facilitates the recombination of two distinct pre-mRNA molecules. Its application for gene therapeutic purposes has been hindered by laborious procedures to identify gene-specific molecules. We have established a screening method for the identification of highly functional RNA trans-splicing molecules based on fluorescence reporters, facilitating the generation of most potent therapeutic molecules for the correction of any gene of interest.Mesh：
Substances：
Year: 2013 PMID： 23325663 DOI： 10.1007/978-1-62703-227-8_30
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745