AIMS AND OBJECTIVE: Mast cells play a central role in allergic and chronic inflammation. Extracts from Clausena lansium (Lour.) Skeels (Rutaceae) possess many pharmacological effects including anti-inflammatory, anti-oxidant, anti-cancer, and anti-trichomonal activities. In addition, the leaves and fruit are used in Chinese folk medicine. We have isolated and identified four known cinnamamides from this plant: lansiumamide C, lansamide I, lansiumamide B, and SB-204900. However, the biological activities of these compounds are not yet understood. The purpose of this paper is to clarify the pharmacological effects of these compounds on mast cells. METHODS: We measured inflammatory molecules in A23187-stimulated rat basophilic leukemia cells (RBL-2H3) treated with these compounds using HPLC, ELISA, and immunoblotting methods. In addition, some signaling molecules were investigated by immunoblotting. RESULTS: Lansamide I, lansiumamide B, and SB-204900 significantly decreased histamine release. Furthermore, lansiumamide B- and SB-204900-treated cells also reduced the protein and/or mRNA levels of TNF-α. SB-204900 markedly suppressed the phosphorylation of p38 MAPK. CONCLUSION: Our findings suggest that lansiumamide B and SB-204900 attenuate mast-cell-induced inflammation.
AIMS AND OBJECTIVE: Mast cells play a central role in allergic and chronic inflammation. Extracts from Clausena lansium (Lour.) Skeels (Rutaceae) possess many pharmacological effects including anti-inflammatory, anti-oxidant, anti-cancer, and anti-trichomonal activities. In addition, the leaves and fruit are used in Chinese folk medicine. We have isolated and identified four known cinnamamides from this plant: lansiumamide C, lansamide I, lansiumamide B, and SB-204900. However, the biological activities of these compounds are not yet understood. The purpose of this paper is to clarify the pharmacological effects of these compounds on mast cells. METHODS: We measured inflammatory molecules in A23187-stimulated rat basophilic leukemia cells (RBL-2H3) treated with these compounds using HPLC, ELISA, and immunoblotting methods. In addition, some signaling molecules were investigated by immunoblotting. RESULTS:Lansamide I, lansiumamide B, and SB-204900 significantly decreased histamine release. Furthermore, lansiumamide B- and SB-204900-treated cells also reduced the protein and/or mRNA levels of TNF-α. SB-204900 markedly suppressed the phosphorylation of p38 MAPK. CONCLUSION: Our findings suggest that lansiumamide B and SB-204900 attenuate mast-cell-induced inflammation.
Authors: Thomas Hochdörfer; Marcel Kuhny; Carolin N Zorn; Rudi W Hendriks; Bart Vanhaesebroeck; Thomas Bohnacker; Gerald Krystal; Michael Huber Journal: Cell Signal Date: 2011-01-22 Impact factor: 4.315
Authors: K Ozawa; Z Szallasi; M G Kazanietz; P M Blumberg; H Mischak; J F Mushinski; M A Beaven Journal: J Biol Chem Date: 1993-01-25 Impact factor: 5.157
Authors: Ana Calheiros de Carvalho; Luiza De Camillis Rodrigues; Alany Ingrid Ribeiro; Maria Fátima das Graças Fernandes da Silva; Lívia Soman de Medeiros; Thiago André Moura Veiga Journal: Molecules Date: 2019-12-30 Impact factor: 4.411