Literature DB >> 23321312

rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells.

Yasuko Onuma1, Hiroaki Tateno, Jun Hirabayashi, Yuzuru Ito, Makoto Asashima.   

Abstract

Cell surface biomarkers have been applied to discriminate pluripotent human embryonic stem cells and induced pluripotent stem cells from differentiated cells. Here, we demonstrate that a recombinant lectin probe, rBC2LCN, a new tool for fluorescence-based imaging and flow cytometry analysis of pluripotent stem cells, is an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent stem cells were visualized in culture medium containing fluorescent dye-labeled rBC2LCN. Fluorescent dye-labeled rBC2LCN was also successfully used to separate live pluripotent stem cells from a mixed cell population by flow cytometry.
Copyright © 2013 Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2013        PMID： 23321312     DOI： 10.1016/j.bbrc.2013.01.025

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  24 in total

Review 1.  Technical approaches to induce selective cell death of pluripotent stem cells.

Authors:  Ho-Chang Jeong; Seung-Ju Cho; Mi-Ok Lee; Hyuk-Jin Cha
Journal:  Cell Mol Life Sci       Date:  2017-02-28       Impact factor: 9.261

2.  Podocalyxin is a glycoprotein ligand of the human pluripotent stem cell-specific probe rBC2LCN.

Authors:  Hiroaki Tateno; Asako Matsushima; Keiko Hiemori; Yasuko Onuma; Yuzuru Ito; Kayo Hasehira; Ken Nishimura; Manami Ohtaka; Satoko Takayasu; Mahito Nakanishi; Yuzuru Ikehara; Mio Nakanishi; Kiyoshi Ohnuma; Techuan Chan; Masashi Toyoda; Hidenori Akutsu; Akihiro Umezawa; Makoto Asashima; Jun Hirabayashi
Journal:  Stem Cells Transl Med       Date:  2013-03-22       Impact factor: 6.940

3.  A stable chimeric fibroblast growth factor (FGF) can successfully replace basic FGF in human pluripotent stem cell culture.

Authors:  Yasuko Onuma; Kumiko Higuchi; Yasuhiko Aiki; Yujing Shu; Masahiro Asada; Makoto Asashima; Masashi Suzuki; Toru Imamura; Yuzuru Ito
Journal:  PLoS One       Date:  2015-04-07       Impact factor: 3.240

4.  Carcinoma-associated fucosylated antigens are markers of the epithelial state and can contribute to cell adhesion through CLEC17A (Prolectin).

Authors:  Adrien Breiman; María Dolores López Robles; Sophie de Carné Trécesson; Klara Echasserieau; Karine Bernardeau; Kurt Drickamer; Anne Imberty; Sophie Barillé-Nion; Frédéric Altare; Jacques Le Pendu
Journal:  Oncotarget       Date:  2016-03-22

5.  Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray.

Authors:  Sayoko Saito; Keiko Hiemori; Kayo Kiyoi; Hiroaki Tateno
Journal:  Sci Rep       Date:  2018-03-05       Impact factor: 4.379

6.  New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells.

Authors:  Carmel M O'Brien; Hun S Chy; Qi Zhou; Shiri Blumenfeld; Jack W Lambshead; Xiaodong Liu; Joshua Kie; Bianca D Capaldo; Tung-Liang Chung; Timothy E Adams; Tram Phan; John D Bentley; William J McKinstry; Karen Oliva; Paul J McMurrick; Yu-Chieh Wang; Fernando J Rossello; Geoffrey J Lindeman; Di Chen; Thierry Jarde; Amander T Clark; Helen E Abud; Jane E Visvader; Christian M Nefzger; Jose M Polo; Jeanne F Loring; Andrew L Laslett
Journal:  Stem Cells       Date:  2017-01-19       Impact factor: 6.277

7.  A selective cytotoxic adenovirus vector for concentration of pluripotent stem cells in human pluripotent stem cell-derived neural progenitor cells.

Authors:  Takamasa Hirai; Ken Kono; Rumi Sawada; Takuya Kuroda; Satoshi Yasuda; Satoko Matsuyama; Akifumi Matsuyama; Naoya Koizumi; Naoki Utoguchi; Hiroyuki Mizuguchi; Yoji Sato
Journal:  Sci Rep       Date:  2021-06-01       Impact factor: 4.379

8.  Elimination of tumorigenic human pluripotent stem cells by a recombinant lectin-toxin fusion protein.

Authors:  Hiroaki Tateno; Yasuko Onuma; Yuzuru Ito; Fumi Minoshima; Sayoko Saito; Madoka Shimizu; Yasuhiko Aiki; Makoto Asashima; Jun Hirabayashi
Journal:  Stem Cell Reports       Date:  2015-04-09       Impact factor: 7.294

9.  A medium hyperglycosylated podocalyxin enables noninvasive and quantitative detection of tumorigenic human pluripotent stem cells.

Authors:  Hiroaki Tateno; Yasuko Onuma; Yuzuru Ito; Keiko Hiemori; Yasuhiko Aiki; Madoka Shimizu; Kumiko Higuchi; Masakazu Fukuda; Masaki Warashina; Susumu Honda; Makoto Asashima; Jun Hirabayashi
Journal:  Sci Rep       Date:  2014-02-12       Impact factor: 4.379

10.  A technique for removing tumourigenic pluripotent stem cells using rBC2LCN lectin.

Authors:  Yoshikazu Haramoto; Yasuko Onuma; Shuuji Mawaribuchi; Yoshiro Nakajima; Yasuhiko Aiki; Kumiko Higuchi; Madoka Shimizu; Hiroaki Tateno; Jun Hirabayashi; Yuzuru Ito
Journal:  Regen Ther       Date:  2020-05-19       Impact factor: 3.419
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.