M Özdemir1, S Yavru, B Baysal. 1. Department of Medical Microbiology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey. mehmetozdem@yahoo.com
Abstract
OBJECTIVE: To investigate the detection of influenza viruses by three different methods. METHODS: Nasopharyngeal swabs were collected from patients with influenza symptoms and examined for influenza A and B viruses using a rapid antigen test, a multiplex polymerase chain reaction (PCR) test and a shell vial cell culture test. RESULTS: Using the shell vial cell culture test, the rapid antigen test and the multiplex PCR test in 130 patients, 31 (23.8%), 24 (18.5%) and 24 (18.5%) samples, respectively, were positive for influenza A and 10 (7.7%), nine (6.9%) and four (3.1%) samples, respectively, were positive for influenza B. Compared with the shell vial test, the sensitivity, specificity, and positive and negative predictive values of the rapid antigen test were 77.4%, 93.3%, 80.0% and 93.1%, respectively, for influenza A, and 90.0%, 95.8%, 64.2% and 99.1%, respectively, for influenza B. The corresponding values for the multiplex PCR test were 77.4%, 95.9%, 85.7% and 93.1%, respectively, for influenza A, and 40.0%, 97.5%, 57.1% and 95.1%, respectively, for influenza B. CONCLUSIONS: The multiplex PCR test and the rapid antigen test are both effective in the detection of influenza A and B viruses.
OBJECTIVE: To investigate the detection of influenza viruses by three different methods. METHODS: Nasopharyngeal swabs were collected from patients with influenza symptoms and examined for influenza A and B viruses using a rapid antigen test, a multiplex polymerase chain reaction (PCR) test and a shell vial cell culture test. RESULTS: Using the shell vial cell culture test, the rapid antigen test and the multiplex PCR test in 130 patients, 31 (23.8%), 24 (18.5%) and 24 (18.5%) samples, respectively, were positive for influenza A and 10 (7.7%), nine (6.9%) and four (3.1%) samples, respectively, were positive for influenza B. Compared with the shell vial test, the sensitivity, specificity, and positive and negative predictive values of the rapid antigen test were 77.4%, 93.3%, 80.0% and 93.1%, respectively, for influenza A, and 90.0%, 95.8%, 64.2% and 99.1%, respectively, for influenza B. The corresponding values for the multiplex PCR test were 77.4%, 95.9%, 85.7% and 93.1%, respectively, for influenza A, and 40.0%, 97.5%, 57.1% and 95.1%, respectively, for influenza B. CONCLUSIONS: The multiplex PCR test and the rapid antigen test are both effective in the detection of influenza A and B viruses.
Authors: John H Powers; Elizabeth D Bacci; Nancy K Leidy; Jiat-Ling Poon; Sonja Stringer; Matthew J Memoli; Alison Han; Mary P Fairchok; Christian Coles; Jackie Owens; Wei-Ju Chen; John C Arnold; Patrick J Danaher; Tahaniyat Lalani; Timothy H Burgess; Eugene V Millar; Michelande Ridore; Andrés Hernández; Patricia Rodríguez-Zulueta; Hilda Ortega-Gallegos; Arturo Galindo-Fraga; Guillermo M Ruiz-Palacios; Sarah Pett; William Fischer; Daniel Gillor; Laura Moreno Macias; Anna DuVal; Richard Rothman; Andrea Dugas; M Lourdes Guerrero Journal: PLoS One Date: 2018-03-22 Impact factor: 3.240