BACKGROUND/AIMS: There is increasing evidence that the PI3K/Akt signal pathway plays a crucial role in tumorigenesis and tumor progression. Akt protein modulates the function of numerous substrates related to the regulation of cell proliferation, such as cyclin dependent kinase inhibitors, including p27 and Cks1. The aim of this study was to investigate the immunohistochemical expressions of Akt, p27 and Cks1 proteins according to clinicopathological parameters such as gender, lymph node metastases, tumor invasion depth, the TNM stage and Lauren classification in gastric adenocarcinoma. METHODOLOGY: Immunohistochemical staining with monoclonal Akt, p27 and Cks1 antibodies was performed on the paraffin embedded specimens of 43 gastric adenocarcinomas. RESULTS: The expression of Akt was increased more in the adenocarcinomas that showed lymph node involvement (53.5%) than in the adenocarcinomas that did not (9.3%) (p=0.007). The expression of Cks1 was increased more in the adenocarcinomas that showed lymph node involvement (39.5%) than in the adenocarcinomas that did not (7%) (p<0.043). CONCLUSIONS: Akt and Cks1 are associated with lymph node metastasis in gastric adenocarcinoma. We suggest that Akt and Cks1 proteins might be related with poor prognosis in gastric adenocarcinoma.
BACKGROUND/AIMS: There is increasing evidence that the PI3K/Akt signal pathway plays a crucial role in tumorigenesis and tumor progression. Akt protein modulates the function of numerous substrates related to the regulation of cell proliferation, such as cyclin dependent kinase inhibitors, including p27 and Cks1. The aim of this study was to investigate the immunohistochemical expressions of Akt, p27 and Cks1 proteins according to clinicopathological parameters such as gender, lymph node metastases, tumor invasion depth, the TNM stage and Lauren classification in gastric adenocarcinoma. METHODOLOGY: Immunohistochemical staining with monoclonal Akt, p27 and Cks1 antibodies was performed on the paraffin embedded specimens of 43 gastric adenocarcinomas. RESULTS: The expression of Akt was increased more in the adenocarcinomas that showed lymph node involvement (53.5%) than in the adenocarcinomas that did not (9.3%) (p=0.007). The expression of Cks1 was increased more in the adenocarcinomas that showed lymph node involvement (39.5%) than in the adenocarcinomas that did not (7%) (p<0.043). CONCLUSIONS:Akt and Cks1 are associated with lymph node metastasis in gastric adenocarcinoma. We suggest that Akt and Cks1 proteins might be related with poor prognosis in gastric adenocarcinoma.
Authors: S V del Rincón; M Widschwendter; D Sun; S Ekholm-Reed; J Tat; L K Teixeira; Z Ellederova; E Grolieres; S I Reed; C Spruck Journal: Oncogene Date: 2014-05-26 Impact factor: 9.867