Literature DB >> 2331483

Stabilization, purification and crystallization of catalytic subunit of cAMP-dependent protein kinase from bovine heart.

S Okuno1, H Fujisawa.   

Abstract

The catalytic subunit of cAMP-dependent protein kinase purified from bovine heart was significantly stabilized by non-ionic detergents. Freezing and thawing did not cause a significant decrease in the enzyme activity in the presence of 0.1% Tween 80. A very convenient method for purification and crystallization of the catalytic subunit was also described.

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Year:  1990        PMID： 2331483     DOI： 10.1016/0167-4838(90)90206-u

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  3 in total

1.  Protein kinase involvement in land snail aestivation and anoxia: protein kinase A kinetic properties and changes in second messenger compounds during depressed metabolism.

Authors:  S P Brooks; K B Storey
Journal:  Mol Cell Biochem       Date:  1996-03-23       Impact factor: 3.396

2.  Increased stabilizing effects of amphiphilic excipients on freeze-drying of lactate dehydrogenase (LDH) by dispersion into sugar matrices.

Authors:  K Izutsu; S Yoshioka; S Kojima
Journal:  Pharm Res       Date:  1995-06       Impact factor: 4.200

3.  cAMP-dependent protein kinase and anoxia survival in turtles: purification and properties of liver PKA.

Authors:  H Mehrani; K B Storey
Journal:  Mol Cell Biochem       Date:  1995-04-12       Impact factor: 3.396
  3 in total

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