Literature DB >> 2331371

Rapid and sensitive detection of DNA in Southern blots with chemiluminescence.

I Bronstein1, J C Voyta, K G Lazzari, O Murphy, B Edwards, L J Kricka.   

Abstract

A new chemiluminescent Southern blot procedure offers molecular biologists a safe, ultrasensitive and rapid alternative to conventional 32P-based systems. This new DNA detection system, SOUTHERN-LIGHT, has been developed by Tropix, Inc. The luminescent signal is produced from a direct chemiluminescent substrate, disodium 3-(4-methoxyspiro[1,2-dioxetane-3-2'-tricyclo-[3.3.1.1 .3,7]decan]-4-yl) phenyl phosphate (AMPPD), which decomposes upon dephosphorylation with alkaline phosphatase. SOUTHERN-LIGHT is an ultrasensitive, rapid detection kit for use with membrane-bound DNA. It is the first test kit to incorporate AMPPD. It requires no specialized equipment and results can be conveniently imaged on instant film or x-ray film within 5-60 min of exposure.

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Year:  1990        PMID： 2331371

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  7 in total

1.  Chemiluminescence detection of proteins from single cells.

Authors:  P G Gillespie; A J Hudspeth
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

2.  Bronchoalveolar lymphocytosis correlates with human T lymphotropic virus type I (HTLV-I) proviral DNA load in HTLV-I carriers.

Authors:  S Mori; A Mizoguchi; M Kawabata; H Fukunaga; K Usuku; I Maruyama; M Osame
Journal:  Thorax       Date:  2005-02       Impact factor: 9.139

3.  Information analysis of Fis binding sites.

Authors:  P N Hengen; S L Bartram; L E Stewart; T D Schneider
Journal:  Nucleic Acids Res       Date:  1997-12-15       Impact factor: 16.971

4.  Non-radioactive detection of oligonucleotide probes by photochemical amplification of dioxetanes.

Authors:  F Schubert; A Knaf; U Möller; D Cech
Journal:  Nucleic Acids Res       Date:  1995-11-25       Impact factor: 16.971

5.  Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene.

Authors:  C Tocharoentanaphol; M Cremer; E Schröck; L Blonden; K Kilian; T Cremer; T Ried
Journal:  Hum Genet       Date:  1994-03       Impact factor: 4.132

6.  Spontaneous in vitro immortalization of breast epithelial cells from a patient with Li-Fraumeni syndrome.

Authors:  J W Shay; G Tomlinson; M A Piatyszek; L S Gollahon
Journal:  Mol Cell Biol       Date:  1995-01       Impact factor: 4.272

7.  Detection of Epstein-Barr virus DNA in anal scrapings from HIV-positive homosexual men.

Authors:  H Näher; B Lenhard; J Wilms; P Nickel
Journal:  Arch Dermatol Res       Date:  1995       Impact factor: 3.017
  7 in total

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