Min-Jung Lee1, Jeong-Oh Shin, Han-Sung Jung. 1. Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Republic of Korea.
Abstract
BACKGROUND: Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains. OBJECTIVE: The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear. METHODS AND RESULTS: In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased. CONCLUSION: These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.
BACKGROUND:Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains. OBJECTIVE: The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear. METHODS AND RESULTS: In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased. CONCLUSION: These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.
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