| Literature DB >> 23306102 |
Tal Gefen1, Jacob Vaya, Soliman Khatib, Natalia Harkevich, Fadi Artoul, E Dan Heller, Jacob Pitcovski, Elina Aizenshtein.
Abstract
Covalent attachment of PEG (PEGylation) is widely used to improve the pharmaceutical properties of therapeutic proteins. The applicability and safety of this method have been proven by the use of various PEGylated pharmaceutical proteins approved by the Food and Drug Administration (FDA). One of the properties attributed to PEGylation is immunogenicity reduction of the PEGylated protein. In this study, the impact of PEGylation on immunogenicity was tested and compared for two proteins (chicken IgY and horse IgG) in two strains of mice (Balb/c and C57BL/6) for two routes of administration (i.v. and i.m.) and two sizes of PEG (5 kD and 20 kD). The influence of PEG was shown to be inconsistent between the mouse strains and routes of administration, even with the same tested protein. Consequently, immunogenicity reduction by PEGylation cannot be predicted or assumed; it must be tested on an individual case basis.Entities:
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Year: 2013 PMID： 23306102 DOI： 10.1016/j.intimp.2012.12.012
Source DB: PubMed Journal: Int Immunopharmacol ISSN： 1567-5769 Impact factor: 4.932