PURPOSE: To evaluate the effect of implant osteotomy on immediate bone cell viability, comparing guided surgery for implant placement with the classic drilling procedure. MATERIALS AND METHODS: For this study, 20 rabbits were used. The animals were divided into a guided surgery group (GG) and a control group (CG) and were then divided into 4 subgroups--subgroups 1, 2, 3, and 4--corresponding to drills used 10, 20, 30, and 40 times, respectively. All animals received 5 osteotomies in each tibia, by use of the classic drilling procedure in one tibia and guided surgery in the other tibia. The osteotomized areas were removed and processed immunohistochemically for detection of osteocalcin, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and caspase 3. RESULTS: Immunohistochemical analysis showed that osteocalcin expression was initially higher in the CG and remained constant after drill reutilization. Although the expressions of RANKL and OPG were not statistically different for the GG and CG, the RANKL/OPG ratio tended to be higher for the GG. Moreover, caspase 3 expression was elevated in the GG, proportionally to the number of osteotomies, indicating an increase in the apoptosis index in the GG. CONCLUSIONS: The classic drilling procedure is more favorable to cell viability than guided surgery.
PURPOSE: To evaluate the effect of implant osteotomy on immediate bone cell viability, comparing guided surgery for implant placement with the classic drilling procedure. MATERIALS AND METHODS: For this study, 20 rabbits were used. The animals were divided into a guided surgery group (GG) and a control group (CG) and were then divided into 4 subgroups--subgroups 1, 2, 3, and 4--corresponding to drills used 10, 20, 30, and 40 times, respectively. All animals received 5 osteotomies in each tibia, by use of the classic drilling procedure in one tibia and guided surgery in the other tibia. The osteotomized areas were removed and processed immunohistochemically for detection of osteocalcin, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and caspase 3. RESULTS: Immunohistochemical analysis showed that osteocalcin expression was initially higher in the CG and remained constant after drill reutilization. Although the expressions of RANKL and OPG were not statistically different for the GG and CG, the RANKL/OPG ratio tended to be higher for the GG. Moreover, caspase 3 expression was elevated in the GG, proportionally to the number of osteotomies, indicating an increase in the apoptosis index in the GG. CONCLUSIONS: The classic drilling procedure is more favorable to cell viability than guided surgery.
Authors: Mariana Raquel da Cruz Vegian; Bruno César Almeida Costa; Gabriela de Fátima Santana-Melo; Fernanda Herrera Costa Godoi; Estela Kaminagakura; Rubens Nisie Tango; Renata Falchete do Prado; Luciane Dias de Oliveira; Claudio Antonio Federico; Sarah de Oliveira Marco Avelino; Rafael Marques Neves; Luana Marotta Reis de Vasconcellos Journal: Clin Oral Investig Date: 2019-06-01 Impact factor: 3.573
Authors: Jônatas Caldeira Esteves; Elcio Marcantonio; Ana Paula de Souza Faloni; Fernanda Regina Godoy Rocha; Rosemary Adriana Marcantonio; Katarzyna Wilk; Giuseppe Intini Journal: J Transl Med Date: 2013-09-23 Impact factor: 5.531