Literature DB >> 23297221

Chemical polysialylation of human recombinant butyrylcholinesterase delivers a long-acting bioscavenger for nerve agents in vivo.

Denis G Ilyushin1, Ivan V Smirnov, Alexey A Belogurov, Igor A Dyachenko, Tatiana Iu Zharmukhamedova, Tatjana I Novozhilova, Eugene A Bychikhin, Marina V Serebryakova, Oleg N Kharybin, Arkadii N Murashev, Konstantin A Anikienko, Eugene N Nikolaev, Natalia A Ponomarenko, Dmitry D Genkin, G Michael Blackburn, Patrick Masson, Alexander G Gabibov.   

Abstract

The creation of effective bioscavengers as a pretreatment for exposure to nerve agents is a challenging medical objective. We report a recombinant method using chemical polysialylation to generate bioscavengers stable in the bloodstream. Development of a CHO-based expression system using genes encoding human butyrylcholinesterase and a proline-rich peptide under elongation factor promoter control resulted in self-assembling, active enzyme multimers. Polysialylation gives bioscavengers with enhanced pharmacokinetics which protect mice against 4.2 LD(50) of S-(2-(diethylamino)ethyl) O-isobutyl methanephosphonothioate without perturbation of long-term behavior.

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Year:  2013        PMID： 23297221      PMCID： PMC3557082          DOI： 10.1073/pnas.1211118110

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  41 in total

1.  Enzyme mimicry by the antiidiotypic antibody approach.

Authors:  A V Kolesnikov; A V Kozyr; E S Alexandrova; F Koralewski; A V Demin; M I Titov; B Avalle; A Tramontano; S Paul; D Thomas; A G Gabibov; A Friboulet
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

2.  Fabrazyme--recombinant protein treatment for Fabry's disease.

Authors:  Debra Barngrover
Journal:  J Biotechnol       Date:  2002-05-23       Impact factor: 3.307

Review 3.  Polysialic acids: potential in improving the stability and pharmacokinetics of proteins and other therapeutics.

Authors:  G Gregoriadis; A Fernandes; M Mital; B McCormack
Journal:  Cell Mol Life Sci       Date:  2000-12       Impact factor: 9.261

4.  Human recombinant DNA-derived antihemophilic factor (factor VIII) in the treatment of hemophilia A. recombinant Factor VIII Study Group.

Authors:  R S Schwartz; C F Abildgaard; L M Aledort; S Arkin; A L Bloom; H H Brackmann; D B Brettler; H Fukui; M W Hilgartner; M J Inwood
Journal:  N Engl J Med       Date:  1990-12-27       Impact factor: 91.245

5.  Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase: expression, purification, characterization and crystallization.

Authors:  Florian Nachon; Yvain Nicolet; Nathalie Viguié; Patrick Masson; Juan C Fontecilla-Camps; Oksana Lockridge
Journal:  Eur J Biochem       Date:  2002-01

6.  Toward antibody-catalyzed hydrolysis of organophosphorus poisons.

Authors:  P Vayron; P Y Renard; F Taran; C Créminon; Y Frobert; J Grassi; C Mioskowski
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-20       Impact factor: 11.205

7.  The proline-rich tetramerization peptides in equine serum butyrylcholinesterase.

Authors:  Kevser Biberoglu; Lawrence M Schopfer; Ozden Tacal; Oksana Lockridge
Journal:  FEBS J       Date:  2012-09-07       Impact factor: 5.542

8.  Wild-type and A328W mutant human butyrylcholinesterase tetramers expressed in Chinese hamster ovary cells have a 16-hour half-life in the circulation and protect mice from cocaine toxicity.

Authors:  Ellen G Duysen; Cynthia F Bartels; Oksana Lockridge
Journal:  J Pharmacol Exp Ther       Date:  2002-08       Impact factor: 4.030

9.  Estimation of the upper limit of human butyrylcholinesterase dose required for protection against organophosphates toxicity: a mathematically based toxicokinetic model.

Authors:  Yacov Ashani; Shlomi Pistinner
Journal:  Toxicol Sci       Date:  2003-11-04       Impact factor: 4.849

10.  Polysialylated insulin: synthesis, characterization and biological activity in vivo.

Authors:  Sanjay Jain; Dale H Hreczuk-Hirst; Brenda McCormack; Malini Mital; Agamemnon Epenetos; Peter Laing; Gregory Gregoriadis
Journal:  Biochim Biophys Acta       Date:  2003-06-20
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  19 in total

1.  Cryo-EM structure of the native butyrylcholinesterase tetramer reveals a dimer of dimers stabilized by a superhelical assembly.

Authors:  Miguel Ricardo Leung; Laura S van Bezouwen; Lawrence M Schopfer; Joel L Sussman; Israel Silman; Oksana Lockridge; Tzviya Zeev-Ben-Mordehai
Journal:  Proc Natl Acad Sci U S A       Date:  2018-12-11       Impact factor: 11.205

2.  Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity.

Authors:  Stanislav S Terekhov; Ivan V Smirnov; Anastasiya V Stepanova; Tatyana V Bobik; Yuliana A Mokrushina; Natalia A Ponomarenko; Alexey A Belogurov; Maria P Rubtsova; Olga V Kartseva; Marina O Gomzikova; Alexey A Moskovtsev; Anton S Bukatin; Michael V Dubina; Elena S Kostryukova; Vladislav V Babenko; Maria T Vakhitova; Alexander I Manolov; Maja V Malakhova; Maria A Kornienko; Alexander V Tyakht; Anna A Vanyushkina; Elena N Ilina; Patrick Masson; Alexander G Gabibov; Sidney Altman
Journal:  Proc Natl Acad Sci U S A       Date:  2017-02-15       Impact factor: 11.205

Review 3.  Cholinesterases and the fine line between poison and remedy.

Authors:  Carey N Pope; Stephen Brimijoin
Journal:  Biochem Pharmacol       Date:  2018-01-31       Impact factor: 5.858

Review 4.  Organophosphate-Hydrolyzing Enzymes as First-Line of Defence Against Nerve Agent-Poisoning: Perspectives and the Road Ahead.

Authors:  A R Satvik Iyengar; Abhay H Pande
Journal:  Protein J       Date:  2016-12       Impact factor: 2.371

5.  Purification of recombinant human butyrylcholinesterase on Hupresin®.

Authors:  Oksana Lockridge; Emilie David; Lawrence M Schopfer; Patrick Masson; Xavier Brazzolotto; Florian Nachon
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2018-10-25       Impact factor: 3.205

6.  Oligomerization status influences subcellular deposition and glycosylation of recombinant butyrylcholinesterase in Nicotiana benthamiana.

Authors:  Jeannine D Schneider; Sylvestre Marillonnet; Alexandra Castilho; Clemens Gruber; Stefan Werner; Lukas Mach; Victor Klimyuk; Tsafrir S Mor; Herta Steinkellner
Journal:  Plant Biotechnol J       Date:  2014-03-11       Impact factor: 9.803

7.  Excessive Labeling Technique Provides a Highly Sensitive Fluorescent Probe for Real-time Monitoring of Biodegradation of Biopolymer Pharmaceuticals in vivo.

Authors:  S S Terekhov; I V Smirnov; O G Shamborant; M A Zenkova; E L Chernolovskaya; D V Gladkikh; A N Murashev; I A Dyachenko; V D Knorre; A A Belogurov; N A Ponomarenko; S M Deyev; V V Vlasov; A G Gabibov
Journal:  Acta Naturae       Date:  2014-10       Impact factor: 1.845

8.  Expression of human butyrylcholinesterase with an engineered glycosylation profile resembling the plasma-derived orthologue.

Authors:  Jeannine D Schneider; Alexandra Castilho; Laura Neumann; Friedrich Altmann; Andreas Loos; Latha Kannan; Tsafrir S Mor; Herta Steinkellner
Journal:  Biotechnol J       Date:  2013-11-08       Impact factor: 4.677

9.  Engineering of complex protein sialylation in plants.

Authors:  Somanath Kallolimath; Alexandra Castilho; Richard Strasser; Clemens Grünwald-Gruber; Friedrich Altmann; Sebastian Strubl; Christina Elisabeth Galuska; Kristina Zlatina; Sebastian Peter Galuska; Stefan Werner; Hauke Thiesler; Sebastian Werneburg; Herbert Hildebrandt; Rita Gerardy-Schahn; Herta Steinkellner
Journal:  Proc Natl Acad Sci U S A       Date:  2016-07-21       Impact factor: 12.779

10.  Chemical Polysialylation and In Vivo Tetramerization Improve Pharmacokinetic Characteristics of Recombinant Human Butyrylcholinesterase-Based Bioscavengers.

Authors:  S S Terekhov; I V Smirnov; O G Shamborant; T V Bobik; D G Ilyushin; A N Murashev; I A Dyachenko; V A Palikov; V D Knorre; A A Belogurov; N A Ponomarenko; E S Kuzina; D D Genkin; P Masson; A G Gabibov
Journal:  Acta Naturae       Date:  2015 Oct-Dec       Impact factor: 1.845
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