Literature DB >> 23295937

Rectal swabs are suitable for quantifying the carriage load of KPC-producing carbapenem-resistant Enterobacteriaceae.

A Lerner1, J Romano, I Chmelnitsky, S Navon-Venezia, R Edgar, Y Carmeli.   

Abstract

It is more convenient and practical to collect rectal swabs than stool specimens to study carriage of colon pathogens. In this study, we examined the ability to use rectal swabs rather than stool specimens to quantify Klebsiella pneumoniae carbapenemase (KPC)-producing carbapenem-resistant Enterobacteriaceae (CRE). We used a quantitative real-time PCR (qPCR) assay to determine the concentration of the bla(KPC) gene relative to the concentration of 16S rRNA genes and a quantitative culture-based method to quantify CRE relative to total aerobic bacteria. Our results demonstrated that rectal swabs are suitable for quantifying the concentration of KPC-producing CRE and that qPCR showed higher correlation between rectal swabs and stool specimens than the culture-based method.

Entities:  

Mesh：

Substances：

Year:  2013        PMID： 23295937      PMCID： PMC3591867          DOI： 10.1128/AAC.01275-12

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  32 in total

1.  Test characteristics of perirectal and rectal swab compared to stool sample for detection of fluoroquinolone-resistant Escherichia coli in the gastrointestinal tract.

Authors:  Ebbing Lautenbach; Anthony D Harris; Eli N Perencevich; Irving Nachamkin; Pam Tolomeo; Joshua P Metlay
Journal:  Antimicrob Agents Chemother       Date:  2005-02       Impact factor: 5.191

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Carbapenem-resistant Enterobacteriaceae: a potential threat.

Authors:  Mitchell J Schwaber; Yehuda Carmeli
Journal:  JAMA       Date:  2008-12-24       Impact factor: 56.272

Review 4.  The changing epidemiology of resistance.

Authors:  Peter M Hawkey; Annie M Jones
Journal:  J Antimicrob Chemother       Date:  2009-09       Impact factor: 5.790

5.  Comparison of rectal swabs and stool cultures in detecting Campylobacter fetus subsp. jejuni.

Authors:  R L Kaplan; L J Goodman; J E Barrett; G M Trenholme; W Landau
Journal:  J Clin Microbiol       Date:  1982-05       Impact factor: 5.948

6.  Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species.

Authors:  V Farrelly; F A Rainey; E Stackebrandt
Journal:  Appl Environ Microbiol       Date:  1995-07       Impact factor: 4.792

7.  Risk factors for new detection of vancomycin-resistant enterococci in acute-care hospitals that employ strict infection control procedures.

Authors:  Alexander A Padiglione; Rory Wolfe; Elizabeth A Grabsch; Di Olden; Stephen Pearson; Clare Franklin; Denis Spelman; Barrie Mayall; Paul D R Johnson; M Lindsay Grayson
Journal:  Antimicrob Agents Chemother       Date:  2003-08       Impact factor: 5.191

8.  Rapid detection of blaKPC carbapenemase genes by real-time PCR.

Authors:  Musa Hindiyeh; Gill Smollen; Zehava Grossman; Daniela Ram; Yehudit Davidson; Fernando Mileguir; Marina Vax; Debbie Ben David; Ilana Tal; Galia Rahav; Ari Shamiss; Ella Mendelson; Nathan Keller
Journal:  J Clin Microbiol       Date:  2008-07-09       Impact factor: 5.948

9.  Quantification of intestinal bacterial populations by real-time PCR with a universal primer set and minor groove binder probes: a global approach to the enteric flora.

Authors:  Stephan J Ott; Meike Musfeldt; Uwe Ullmann; Jochen Hampe; Stefan Schreiber
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

10.  Molecular and descriptive epidemiologyof multidrug-resistant Enterobacteriaceae in hospitalized infants.

Authors:  Brian Anderson; Sarah Nicholas; Bruce Sprague; Joseph Campos; Billie Short; Nalini Singh
Journal:  Infect Control Hosp Epidemiol       Date:  2008-03       Impact factor: 3.254
View more
  20 in total

Review 1.  Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods.

Authors:  Roberto Viau; Karen M Frank; Michael R Jacobs; Brigid Wilson; Keith Kaye; Curtis J Donskey; Federico Perez; Andrea Endimiani; Robert A Bonomo
Journal:  Clin Microbiol Rev       Date:  2016-01       Impact factor: 26.132

2.  Real-time PCR threshold cycle cutoffs help to identify agents causing acute childhood diarrhea in Zanzibar.

Authors:  Kristina Elfving; Maria Andersson; Mwinyi I Msellem; Christina Welinder-Olsson; Max Petzold; Anders Björkman; Birger Trollfors; Andreas Mårtensson; Magnus Lindh
Journal:  J Clin Microbiol       Date:  2014-01-08       Impact factor: 5.948

3.  Amplification of Antimicrobial Resistance in Gut Flora of Patients Treated with Ceftriaxone.

Authors:  J Meletiadis; A Turlej-Rogacka; A Lerner; A Adler; E Tacconelli; J W Mouton
Journal:  Antimicrob Agents Chemother       Date:  2017-10-24       Impact factor: 5.191

4.  Relative fecal abundance of extended-spectrum-β-lactamase-producing Escherichia coli strains and their occurrence in urinary tract infections in women.

Authors:  Etienne Ruppé; Brandusa Lixandru; Radu Cojocaru; Cagri Büke; Elisabeth Paramythiotou; Cécile Angebault; Claire Visseaux; Ingrid Djuikoue; Esra Erdem; Olga Burduniuc; Assiya El Mniai; Candice Marcel; Marion Perrier; Thomas Kesteman; Olivier Clermont; Erick Denamur; Laurence Armand-Lefèvre; Antoine Andremont
Journal:  Antimicrob Agents Chemother       Date:  2013-07-08       Impact factor: 5.191

5.  A prospective study in severely injured patients reveals an altered gut microbiome is associated with transfusion volume.

Authors:  Susannah E Nicholson; David M Burmeister; Taylor R Johnson; Yi Zou; Zhao Lai; Shannon Scroggins; Mark DeRosa; Rachelle B Jonas; Daniel R Merrill; Caroline Zhu; Larry M Newton; Ronald M Stewart; Martin G Schwacha; Donald H Jenkins; Brian J Eastridge
Journal:  J Trauma Acute Care Surg       Date:  2019-04       Impact factor: 3.313

6.  Mortality in Thai Nursing Homes Based on Antimicrobial-Resistant Enterobacterales Carriage and COVID-19 Lockdown Timing: A Prospective Cohort Study.

Authors:  Thundon Ngamprasertchai; Muthita Vanaporn; Sant Muangnoicharoen; Wirichada Pan-Ngum; Narisa Ruenroengbun; Pittaya Piroonamornpun; Thitiya Ponam; Chatnapa Duangdee; Phanita Chankete; Anupop Jitmuang; Visanu Thamlikitkul
Journal:  Antibiotics (Basel)       Date:  2022-06-02

7.  The pros, cons, and unknowns of search and destroy for carbapenem-resistant enterobacteriaceae.

Authors:  Prashini Moodley; Andrew Whitelaw
Journal:  Curr Infect Dis Rep       Date:  2015-06       Impact factor: 3.725

8.  Comparison of rectal swabs and faeces for real-time PCR detection of enteric agents in Rwandan children with gastroenteritis.

Authors:  Jean-Claude Kabayiza; Maria E Andersson; Christina Welinder-Olsson; Tomas Bergström; Gregoire Muhirwa; Magnus Lindh
Journal:  BMC Infect Dis       Date:  2013-09-27       Impact factor: 3.090

9.  Metabolomics analysis identifies intestinal microbiota-derived biomarkers of colonization resistance in clindamycin-treated mice.

Authors:  Robin L P Jump; Alex Polinkovsky; Kelly Hurless; Brett Sitzlar; Kevin Eckart; Myreen Tomas; Abhishek Deshpande; Michelle M Nerandzic; Curtis J Donskey
Journal:  PLoS One       Date:  2014-07-02       Impact factor: 3.240

10.  Causes, consequences, and perspectives in the variations of intestinal density of colonization of multidrug-resistant enterobacteria.

Authors:  Etienne Ruppé; Antoine Andremont
Journal:  Front Microbiol       Date:  2013-05-28       Impact factor: 5.640
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.