INTRODUCTION: Toxoplasma gondii is an intracellular parasite that causes severe disease when the infection occurs during pregnancy. Trophoblast cells constitute an important maternal-fetal barrier, with monocytes concentrating around them. Thus, interactions between trophoblasts and monocytes are important for maintaining a successful pregnancy, especially in cases of infection. This study aimed to evaluate the role of trophoblast cells (BeWo line) on monocyte (THP-1 line) activity in the presence or absence of T. gondii infection. METHODS: THP-1 cells were stimulated with supernatants of BeWo cells, previously infected or not with T. gondii, and then infected with parasites. The supernatant of both cells were collected and analyzed for cytokine production and T. gondii proliferation in THP-1 cells was determined. RESULTS: The results showed that after infection, the pattern of cytokines secreted by THP-1 and BeWo cells was characterized as a pro-inflammatory profile. Furthermore, supernatant of BeWo cells infected or not, was able to change the cytokine profile secreted by infected THP-1 cells, and this supernatant became THP-1 cells more able to control T. gondii proliferation than those that had not been stimulated. DISCUSSION: This effect was associated with secretion of interleukin (IL)-6 by the THP-1 cells and soluble factors secreted by BeWo cells, such as IL-6 and MIF. CONCLUSION: Together, these results suggest that trophoblast cells are able to modulate monocyte activity, resulting in the control of T. gondii infection and subsequent maintenance of pregnancy.
INTRODUCTION: Toxoplasma gondii is an intracellular parasite that causes severe disease when the infection occurs during pregnancy. Trophoblast cells constitute an important maternal-fetal barrier, with monocytes concentrating around them. Thus, interactions between trophoblasts and monocytes are important for maintaining a successful pregnancy, especially in cases of infection. This study aimed to evaluate the role of trophoblast cells (BeWo line) on monocyte (THP-1 line) activity in the presence or absence of T. gondii infection. METHODS:THP-1 cells were stimulated with supernatants of BeWo cells, previously infected or not with T. gondii, and then infected with parasites. The supernatant of both cells were collected and analyzed for cytokine production and T. gondii proliferation in THP-1 cells was determined. RESULTS: The results showed that after infection, the pattern of cytokines secreted by THP-1 and BeWo cells was characterized as a pro-inflammatory profile. Furthermore, supernatant of BeWo cells infected or not, was able to change the cytokine profile secreted by infected THP-1 cells, and this supernatant became THP-1 cells more able to control T. gondii proliferation than those that had not been stimulated. DISCUSSION: This effect was associated with secretion of interleukin (IL)-6 by the THP-1 cells and soluble factors secreted by BeWo cells, such as IL-6 and MIF. CONCLUSION: Together, these results suggest that trophoblast cells are able to modulate monocyte activity, resulting in the control of T. gondii infection and subsequent maintenance of pregnancy.
Authors: Lukasz Przybyl; Nadine Haase; Michaela Golic; Julianna Rugor; Maria Emilia Solano; Petra Clara Arck; Martin Gauster; Berthold Huppertz; Christoph Emontzpohl; Christian Stoppe; Jürgen Bernhagen; Lin Leng; Richard Bucala; Herbert Schulz; Arnd Heuser; M Susanne Weedon-Fekjær; Guro M Johnsen; Dirk Peetz; Friedrich C Luft; Anne Cathrine Staff; Dominik N Müller; Ralf Dechend; Florian Herse Journal: Circ Res Date: 2016-05-19 Impact factor: 17.367
Authors: Rafaela J da Silva; Angelica O Gomes; Priscila S Franco; Ariane S Pereira; Iliana C B Milian; Mayara Ribeiro; Paolo Fiorenzani; Maria C Dos Santos; José R Mineo; Neide M da Silva; Eloisa A V Ferro; Bellisa de Freitas Barbosa Journal: Front Cell Infect Microbiol Date: 2017-07-26 Impact factor: 5.293
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Authors: Letícia S Castro-Filice; Bellisa F Barbosa; Mariana B Angeloni; Neide M Silva; Angelica O Gomes; Celene M O S Alves; Deise A O Silva; Olindo A Martins-Filho; Maria C Santos; José R Mineo; Eloisa A V Ferro Journal: J Transl Med Date: 2014-05-19 Impact factor: 5.531