NTA-modified carbon electrode as a general relaying substrate to facilitate electron transfer of SOD: application to in vivo monitoring of O2(-) in a rat brain.

Nitrilotriacetic acid (NTA)/histidine-tag (HT) technology has been first employed to facilitate the electron transfer of superoxide dismutase (SOD), and further been developed for in vivo monitoring of superoxide anion (O2(-)) in a rat brain during cerebral ischemia/reperfusion processes. Direct electron transfer of SOD is greatly enhanced at NTA-modified electrode with a high rate constant (ks) of 24±1.1s(-1). The formal potential (E(0)') of SOD is estimated to be 5±2.5mV vs. Ag