| Literature DB >> 2328905 |
T Ezaki1, S M Saidi, S L Liu, Y Hashimoto, H Yamamoto, E Yabuuchi.
Abstract
A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromo-peptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h.Entities:
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Year: 1990 PMID： 2328905 DOI： 10.1016/0378-1097(90)90180-x
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742