Mutational analysis of the three tandem domains of ε-poly-L-lysine synthetase catalyzing the L-lysine polymerization reaction.

ε-Poly-l-lysine (ε-PL) synthetase (Pls) is a nonribosomal peptide synthetase (NRPS)-like enzyme with three tandem domains to catalyze the l-lysine polymerization reaction. Mutational analysis of the three tandem domains demonstrated that the interconnected action of all three domains is essential for the enzyme activity.