BACKGROUND: This study determines the protein carbonyls which cause cellular damage and glutathione, glutathione peroxidase, glutathione reductase act as antioxidants. MATERIALS AND METHODS: This study was carried out in different categories of pulmonary and extra pulmonary tuberculosis cases of newly sputum culture positive diagnosed pulmonary categorie I (n=100), extra pulmonary patients categorie (n=35) before and after the DOTS treatment of 6 months, categorie II (n=100), categorie III (n=100) and in normal control subjects (n=100). RESULTS: The serum protein carbonyl levels were significantly increased in the pulmonary and extra pulmonary tuberculosis patients. The activities of blood glutathione, glutathione peroxidase, and glutathione reductase were found to be significantly decreased in subjects of all the categories of pulmonary and extra pulmonary tuberculosis. A negative correlation between the carbonyl protein content and glutathione, glutathione peroxidase, and glutathione reductase was seen in pulmonary tuberculosis, p<0.001. CONCLUSION: Increased antioxidant defense mechanism due to increase oxidative stress in tuberculosis. The changes were reversed after 6 months of antitubercular treatment in patients with a good recovery, but the increase in the oxidative stress was not completely reversed.
BACKGROUND: This study determines the protein carbonyls which cause cellular damage and glutathione, glutathione peroxidase, glutathione reductase act as antioxidants. MATERIALS AND METHODS: This study was carried out in different categories of pulmonary and extra pulmonary tuberculosis cases of newly sputum culture positive diagnosed pulmonary categorie I (n=100), extra pulmonary patients categorie (n=35) before and after the DOTS treatment of 6 months, categorie II (n=100), categorie III (n=100) and in normal control subjects (n=100). RESULTS: The serum protein carbonyl levels were significantly increased in the pulmonary and extra pulmonary tuberculosispatients. The activities of blood glutathione, glutathione peroxidase, and glutathione reductase were found to be significantly decreased in subjects of all the categories of pulmonary and extra pulmonary tuberculosis. A negative correlation between the carbonyl protein content and glutathione, glutathione peroxidase, and glutathione reductase was seen in pulmonary tuberculosis, p<0.001. CONCLUSION: Increased antioxidant defense mechanism due to increase oxidative stress in tuberculosis. The changes were reversed after 6 months of antitubercular treatment in patients with a good recovery, but the increase in the oxidative stress was not completely reversed.
Entities:
Keywords:
Carbonyl protein content; GSH-Px; GSH-Rx; Glutathione; Tuberculosis
Authors: R L Levine; D Garland; C N Oliver; A Amici; I Climent; A G Lenz; B W Ahn; S Shaltiel; E R Stadtman Journal: Methods Enzymol Date: 1990 Impact factor: 1.600
Authors: J A De Andrade; J P Crow; L Viera; C Bruce Alexander; K Randall Young; D C McGiffin; G L Zorn; S Zhu; S Matalon; R M Jackson Journal: Am J Respir Crit Care Med Date: 2000-06 Impact factor: 21.405