PURPOSE: Staphylococcus aureus and Pseudomonas aeruginosa are frequently isolated from biofilm-associated orthopedic implant infections. This study assessed the activity of N-acetyl-L-cysteine at inhibitory and subinhibitory concentrations against biofilm formed by S. aureus and P. aeruginosa on prosthetic materials. METHODS: S. aureus and P. aeruginosa biofilm on smooth polyethylene and sand-blasted titanium discs was determined by means of a spectrophotometric assay before and at 3, 6, and 18 hours after addition of N-acetyl-L-cysteine. RESULTS: N-acetyl-L-cysteine was able to disaggregate biofilm produced by S. aureus and P. aeruginosa on polyethylene and titanium discs after 3 h of incubation. A different rate in eradication of biofilm produced by P. aeruginosa was seen between polyethylene and titanium (50% vs. 20% respectively vs. biofilm formed before treatment). Results were confirmed by confocal laser scanning microscopy. CONCLUSIONS: The results suggest a possible role of N-acetyl-L-cysteine in the treatment of orthopedic prosthetic infections.
PURPOSE:Staphylococcus aureus and Pseudomonas aeruginosa are frequently isolated from biofilm-associated orthopedic implant infections. This study assessed the activity of N-acetyl-L-cysteine at inhibitory and subinhibitory concentrations against biofilm formed by S. aureus and P. aeruginosa on prosthetic materials. METHODS:S. aureus and P. aeruginosa biofilm on smooth polyethylene and sand-blasted titanium discs was determined by means of a spectrophotometric assay before and at 3, 6, and 18 hours after addition of N-acetyl-L-cysteine. RESULTS:N-acetyl-L-cysteine was able to disaggregate biofilm produced by S. aureus and P. aeruginosa on polyethylene and titanium discs after 3 h of incubation. A different rate in eradication of biofilm produced by P. aeruginosa was seen between polyethylene and titanium (50% vs. 20% respectively vs. biofilm formed before treatment). Results were confirmed by confocal laser scanning microscopy. CONCLUSIONS: The results suggest a possible role of N-acetyl-L-cysteine in the treatment of orthopedic prosthetic infections.
Authors: Lorenzo Drago; Willemijn Boot; Kostantinos Dimas; Kostantinos Malizos; Gertrud M Hänsch; Jos Stuyck; Debby Gawlitta; Carlo L Romanò Journal: Clin Orthop Relat Res Date: 2014-11 Impact factor: 4.176
Authors: Fabíola Costa; Daniela M Sousa; Paula Parreira; Meriem Lamghari; Paula Gomes; M Cristina L Martins Journal: Sci Rep Date: 2017-12-12 Impact factor: 4.379