Raziye Tajalli1,2, Maliheh Nobakht1,3, Hajar Mohammadi-Barzelighi1, Shahram Agah4, Abdolaziz Rastegar-Lari1, Alireza Sadeghipour5. 1. Anti-Microbial Resistance Research Center, Tehran University of Medical Sciences, Tehran, Iran. 2. Science and Research Branch, Islamic Azad university, Tehran, Iran. 3. Dept. of Histology and Neuroscience, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 4. Colorectal Research Center, Tehran University of Medical Sciences, Tehran; 5Dep. of Oncopathology, Tehran University of Medical Sciences, Tehran, Iran. 5. Dep. of Oncopathology, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Helicobacter pylori, which is associated with many upper gastrointestinal diseases, is found in half of the population of the world. Several special stains and immunohistochemistry stain for H. pylori are available. The need for and usefulness of immunohistochemical (IHC) technique has been debated for years. Toluidine blue is a simple stain for microbiological studies and is easily available in laboratories. Therefore, this study was conducted to compare hematoxylin and eosin (H&E), Giemsa and toluidine blue staining with immunehistochemistry for detection of H. pylori in patients with gastritis and also to correlate the results of these staining methods with pathological grading. METHODS: We reviewed 54 consecutive gastric biopsy specimens stained by H&E and Giemsa as well as by toluidine blue and immunohistochemistry stains for H. pylori. RESULTS: H. pylori was positively identified by IHC in 43 (79.63%) patients, while positive samples were found in 18 (33.33%), 24 (44.44%) and 33 (61.11%) patients using H&E, Giemsa and toluidine blue staining methods. Our results showed that classical histological staining methods are not sensitive enough to identify low numbers or coccoid forms of organism, while toluidine blue and immunohistochemistry play an important role in detection of H. pylori infection. CONCLUSION: Toluidine blue has been proved to be much more reliable than H&E and Giemsa in detection of H. pylori. In addition, in post treatment biopsies and in biopsies with unexplained chronic active gastritis without histological evidence of H. pylori should have immunohistochemistry done to detect possible low density or coccoid form of organisms.
BACKGROUND:Helicobacter pylori, which is associated with many upper gastrointestinal diseases, is found in half of the population of the world. Several special stains and immunohistochemistry stain for H. pylori are available. The need for and usefulness of immunohistochemical (IHC) technique has been debated for years. Toluidine blue is a simple stain for microbiological studies and is easily available in laboratories. Therefore, this study was conducted to compare hematoxylin and eosin (H&E), Giemsa and toluidine blue staining with immunehistochemistry for detection of H. pylori in patients with gastritis and also to correlate the results of these staining methods with pathological grading. METHODS: We reviewed 54 consecutive gastric biopsy specimens stained by H&E and Giemsa as well as by toluidine blue and immunohistochemistry stains for H. pylori. RESULTS:H. pylori was positively identified by IHC in 43 (79.63%) patients, while positive samples were found in 18 (33.33%), 24 (44.44%) and 33 (61.11%) patients using H&E, Giemsa and toluidine blue staining methods. Our results showed that classical histological staining methods are not sensitive enough to identify low numbers or coccoid forms of organism, while toluidine blue and immunohistochemistry play an important role in detection of H. pyloriinfection. CONCLUSION:Toluidine blue has been proved to be much more reliable than H&E and Giemsa in detection of H. pylori. In addition, in post treatment biopsies and in biopsies with unexplained chronic active gastritis without histological evidence of H. pylori should have immunohistochemistry done to detect possible low density or coccoid form of organisms.
Authors: B D Gold; R B Colletti; M Abbott; S J Czinn; Y Elitsur; E Hassall; C Macarthur; J Snyder; P M Sherman Journal: J Pediatr Gastroenterol Nutr Date: 2000-11 Impact factor: 2.839
Authors: Jason Weiss; Tat-Kin Tsang; Xiangwen Meng; H Zhang; Elizabeth Kilner; Edward Wang; William Watkin Journal: Am J Clin Pathol Date: 2008-01 Impact factor: 2.493