| Literature DB >> 23279585 |
Abstract
Simultaneous overexpression of an optimized, cytosolically localized valine biosynthesis pathway together with overexpression of xylose isomerase XylA from Clostridium phytofermentans, transaldolase Tal1 and xylulokinase Xks1 enabled recombinant Saccharomyces cerevisiae cells to complement the valine auxotrophy of ilv2,3,5 triple deletion mutants for growth on D-xylose as the sole carbon source. Moreover, after additional overexpression of ketoacid decarboxylase Aro10 and alcohol dehydrogenase Adh2, the cells were able to ferment D-xylose directly to isobutanol.Entities:
Mesh:
Substances:
Year: 2013 PMID: 23279585 DOI: 10.1111/1567-1364.12028
Source DB: PubMed Journal: FEMS Yeast Res ISSN: 1567-1356 Impact factor: 2.796