PROBLEM: There is a strong correlation between intrauterine bacterial infection and preterm labor. While inflammation is a common mechanism, certain pathogens may trigger placental apoptosis. TLR2 activation by gram-positive bacterial peptidoglycan (PDG) induces first-trimester trophoblast apoptosis and decreased IL-6 secretion. This is dependent upon the presence of TLR1 and the absence of TLR6 and both TLR2 coreceptors. As TLR10 is also a TLR2 coreceptor, the objective of this study was to determine its expression and function in the trophoblast. METHOD OF STUDY: First-and third-trimester human placental tissue and isolated trophoblast were evaluated for TLR10 expression. A first-trimester human trophoblast cell line stably transfected with a TLR10 dominant negative (TLR10-DN) or vector control was treated with or without PDG and analyzed for apoptosis and IL-6. RESULTS: TLR10 was expressed by trophoblasts during the first and third trimesters of pregnancy. PDG-induced trophoblast caspase-3 activity was inhibited by the presence of the TLR10-DN. The presence of the TLR10-DN had no effect on PDG reduction in trophoblast IL-6 secretion. CONCLUSION: This study demonstrates that trophoblast TLR10 plays a role in promoting apoptosis triggered by gram-positive bacterial components and suggests that TLR10 may regulate the balance between trophoblast survival and cell death.
PROBLEM: There is a strong correlation between intrauterine bacterial infection and preterm labor. While inflammation is a common mechanism, certain pathogens may trigger placental apoptosis. TLR2 activation by gram-positive bacterial peptidoglycan (PDG) induces first-trimester trophoblast apoptosis and decreased IL-6 secretion. This is dependent upon the presence of TLR1 and the absence of TLR6 and both TLR2 coreceptors. As TLR10 is also a TLR2 coreceptor, the objective of this study was to determine its expression and function in the trophoblast. METHOD OF STUDY: First-and third-trimester human placental tissue and isolated trophoblast were evaluated for TLR10 expression. A first-trimester human trophoblast cell line stably transfected with a TLR10 dominant negative (TLR10-DN) or vector control was treated with or without PDG and analyzed for apoptosis and IL-6. RESULTS:TLR10 was expressed by trophoblasts during the first and third trimesters of pregnancy. PDG-induced trophoblast caspase-3 activity was inhibited by the presence of the TLR10-DN. The presence of the TLR10-DN had no effect on PDG reduction in trophoblast IL-6 secretion. CONCLUSION: This study demonstrates that trophoblast TLR10 plays a role in promoting apoptosis triggered by gram-positive bacterial components and suggests that TLR10 may regulate the balance between trophoblast survival and cell death.
Authors: Roberto Romero; Jimmy Espinoza; Luís F Gonçalves; Juan Pedro Kusanovic; Lara Friel; Sonia Hassan Journal: Semin Reprod Med Date: 2007-01 Impact factor: 1.303
Authors: Shalini Patni; Louise P Wynen; Anna L Seager; Gareth Morgan; John O White; Catherine A Thornton Journal: Biol Reprod Date: 2008-09-24 Impact factor: 4.285
Authors: Yue Guan; Diana Rose E Ranoa; Song Jiang; Sarita K Mutha; Xinyan Li; Jerome Baudry; Richard I Tapping Journal: J Immunol Date: 2010-03-26 Impact factor: 5.422
Authors: John V Ilekis; Ekaterini Tsilou; Susan Fisher; Vikki M Abrahams; Michael J Soares; James C Cross; Stacy Zamudio; Nicholas P Illsley; Leslie Myatt; Christine Colvis; Maged M Costantine; David M Haas; Yoel Sadovsky; Carl Weiner; Erik Rytting; Gene Bidwell Journal: Am J Obstet Gynecol Date: 2016-03-10 Impact factor: 8.661
Authors: Mai Hoang; Julie A Potter; Stefan M Gysler; Christina S Han; Seth Guller; Errol R Norwitz; Vikki M Abrahams Journal: Biol Reprod Date: 2014-02-27 Impact factor: 4.285
Authors: Marije Oosting; Shih-Chin Cheng; Judith M Bolscher; Rachel Vestering-Stenger; Theo S Plantinga; Ineke C Verschueren; Peer Arts; Anja Garritsen; Hans van Eenennaam; Patrick Sturm; Bart-Jan Kullberg; Alexander Hoischen; Gosse J Adema; Jos W M van der Meer; Mihai G Netea; Leo A B Joosten Journal: Proc Natl Acad Sci U S A Date: 2014-10-06 Impact factor: 11.205
Authors: Ruoyi Jiang; Hailong Meng; Khadir Raddassi; Ira Fleming; Kenneth B Hoehn; Kenneth R Dardick; Alexia A Belperron; Ruth R Montgomery; Alex K Shalek; David A Hafler; Steven H Kleinstein; Linda K Bockenstedt Journal: JCI Insight Date: 2021-06-22