Literature DB >> 2327564

Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity.

C R Wheeler1, J A Salzman, N M Elsayed, S T Omaye, D W Korte.   

Abstract

Automated assays for catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase are presented. The assay for catalase is based on the peroxidatic activity of the enzyme. The glutathione peroxidase and reductase assays measure the consumption of NADPH following the reduction of t-butyl hydroperoxide and oxidized glutathione, respectively. The assay for superoxide dismutase is based on the reduction of cytochrome c. All assays utilize the Cobas FARA clinical automated analyzer and provide considerable time savings over the manual assays.

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Year:  1990        PMID: 2327564     DOI: 10.1016/0003-2697(90)90668-y

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  121 in total

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Journal:  Age (Dordr)       Date:  2012-01-04

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4.  Cellular responses required for oxidative stress tolerance, colonization, and lesion formation by the necrotrophic fungus Alternaria alternata in citrus.

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Journal:  Curr Microbiol       Date:  2010-10-27       Impact factor: 2.188

5.  Crepidiastrum denticulatum extract protects the liver against chronic alcohol-induced damage and fat accumulation in rats.

Authors:  Ji-Hye Yoo; Kyungsu Kang; Ji Ho Yun; Mi Ae Kim; Chu Won Nho
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6.  Dopamine as a potent inducer of cellular glutathione and NAD(P)H:quinone oxidoreductase 1 in PC12 neuronal cells: a potential adaptive mechanism for dopaminergic neuroprotection.

Authors:  Zhenquan Jia; Hong Zhu; Bhaba R Misra; Yunbo Li; Hara P Misra
Journal:  Neurochem Res       Date:  2008-03-27       Impact factor: 3.996

7.  In vivo bioassays of acute asbestosis and its correlation with ESR spectroscopy and imaging in redox status.

Authors:  Stephen S Leonard; Kristina Mowrey; Donna Pack; Xianglin Shi; Vince Castranova; Periannan Kuppusamy; Val Vallyathan
Journal:  Mol Cell Biochem       Date:  2002 May-Jun       Impact factor: 3.396

8.  Exercise-induced brachial artery blood flow and vascular function is impaired in systemic sclerosis.

Authors:  Daniel R Machin; Heather L Clifton; Ryan S Garten; Jayson R Gifford; Russell S Richardson; D Walter Wray; Tracy M Frech; Anthony J Donato
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9.  Quinolinic Acid-Induced Huntington Disease-Like Symptoms Mitigated by Potent Free Radical Scavenger Edaravone-a Pilot Study on Neurobehavioral, Biochemical, and Histological Approach in Male Wistar Rats.

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10.  Activated peroxisomal fatty acid metabolism improves cardiac recovery in ischemia-reperfusion.

Authors:  Edgars Liepinsh; Elina Skapare; Janis Kuka; Marina Makrecka; Helena Cirule; Edijs Vavers; Eduards Sevostjanovs; Solveiga Grinberga; Osvalds Pugovics; Maija Dambrova
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2013-03-26       Impact factor: 3.000

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