Literature DB >> 23275136

The MTT assay is a rapid and reliable quantitative method to assess Staphylococcus aureus induced endothelial cell damage.

Kati Seidl1, Annelies S Zinkernagel.   

Abstract

We established the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess damage induced by Staphylococcus aureus in human umbilical vein endothelial cells (HUVECs). The MTT assay was comparable to the previously published (51)chromium release assay. MTT reduction by intracellular S. aureus was negligible and therefore permits assessment of S. aureus induced EC damage.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 23275136     DOI： 10.1016/j.mimet.2012.12.018

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  12 in total

1.  MgrA Governs Adherence, Host Cell Interaction, and Virulence in a Murine Model of Bacteremia Due to Staphylococcus aureus.

Authors:  Liang Li; Genzhu Wang; Ambrose Cheung; Wessam Abdelhady; Kati Seidl; Yan Q Xiong
Journal:  J Infect Dis       Date:  2019-08-09       Impact factor: 5.226

2.  The ArlRS two-component system is a regulator of Staphylococcus aureus-induced endothelial cell damage.

Authors:  Kati Seidl; Michèle Leemann; Annelies S Zinkernagel
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-11-27       Impact factor: 3.267

3.  Apoptotic effects of Photofrin-Diomed 630-PDT on SHEEC human esophageal squamous cancer cells.

Authors:  Shegan Gao; Mengxi Zhang; Xiaojuan Zhu; Zhifeng Qu; Tanyou Shan; Xuanhu Xie; Ying Wang; Xiaoshan Feng
Journal:  Int J Clin Exp Med       Date:  2015-09-15

4.  Nanocurcumin protects cardiomyoblasts H9c2 from hypoxia-induced hypertrophy and apoptosis by improving oxidative balance.

Authors:  Sarita Nehra; Varun Bhardwaj; Namita Kalra; Lilly Ganju; Anju Bansal; Shweta Saxena; Deepika Saraswat
Journal:  J Physiol Biochem       Date:  2015-04-07       Impact factor: 4.158

5.  The Stringent Response Contributes to Persistent Methicillin-Resistant Staphylococcus aureus Endovascular Infection Through the Purine Biosynthetic Pathway.

Authors:  Liang Li; Arnold S Bayer; Ambrose Cheung; Lou Lu; Wessam Abdelhady; Niles P Donegan; Jong-In Hong; Michael R Yeaman; Yan Q Xiong
Journal:  J Infect Dis       Date:  2020-09-01       Impact factor: 5.226

6.  Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro.

Authors:  Di Wu; Yana Zhao; Shengnan Fu; Jianbo Zhang; Wenhang Wang; Zhexian Yan; Heng Guo; Anjun Liu
Journal:  Cell Cycle       Date:  2018-08-02       Impact factor: 4.534

7.  Secretome analysis defines the major role of SecDF in Staphylococcus aureus virulence.

Authors:  Chantal Quiblier; Kati Seidl; Bernd Roschitzki; Annelies S Zinkernagel; Brigitte Berger-Bächi; Maria M Senn
Journal:  PLoS One       Date:  2013-05-03       Impact factor: 3.240

8.  Novel near-infrared fluorescent probe for live cell imaging.

Authors:  Meng Wan; Yubing Zhu; Jianjun Zou
Journal:  Exp Ther Med       Date:  2019-12-13       Impact factor: 2.447

9.  Protective Effect of Ginkgolic Acid in Attenuating LDL Induced Inflammation Human Peripheral Blood Mononuclear Cells via Altering the NF-κB Signaling Pathway.

Authors:  Juan Zhang; Jifeng Yan
Journal:  Front Pharmacol       Date:  2019-11-08       Impact factor: 5.810

10.  Targeting Hidden Pathogens: Cell-Penetrating Enzybiotics Eradicate Intracellular Drug-Resistant Staphylococcus aureus.

Authors:  Christian Röhrig; Markus Huemer; Dominique Lorgé; Samuel Luterbacher; Preeda Phothaworn; Christopher Schefer; Anna M Sobieraj; Léa V Zinsli; Srikanth Mairpady Shambat; Nadja Leimer; Anja P Keller; Fritz Eichenseher; Yang Shen; Sunee Korbsrisate; Annelies S Zinkernagel; Martin J Loessner; Mathias Schmelcher
Journal:  mBio       Date:  2020-04-14       Impact factor: 7.867
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