BACKGROUND: An understanding of causal relations between diet and health is hindered by the lack of robust biological markers of food exposure. OBJECTIVE: We aimed to develop a data-driven procedure to discover urine biomarkers indicative of habitual exposure to different foods. DESIGN: The habitual diet of 68 participants was assessed by using 4 food-frequency questionnaires over 3 mo, and participants were assigned to different consumption-frequency classes for 58 dietary components. Flow infusion electrospray-ionization mass spectrometry followed by supervised multivariate data analysis was used to determine whether the chemical composition of urine was related to specific differences in the consumption levels of each food. RESULTS: Foods were eaten habitually in 1 of 5 basic patterns differing in range and distribution of consumption frequency. Overnight, 24-h, and fasting urine samples proved useful for biomarker lead discovery with habitual citrus exposure used as a paradigm. Exposure level discrimination robustness improved linearly as urine samples from low-frequency citrus consumers were compared with urine samples from participants reporting increasingly higher intakes. For all foods, distinctiveness and consumption-frequency range influenced the likelihood that differential dietary exposure could be detected. Model output statistics indicated foods for which biomarker lead discovery was feasible. Metabolites proposed previously as acute intake biomarkers of citrus (proline betaine), oily fish (methylhistidine), coffee (dihydrocaffeic acid derivatives), and tomato (phenolic metabolites) were also biomarkers of habitual exposure. A significance threshold in modeling output statistics was determined to guide the discovery of potential biomarkers for other foods. CONCLUSION: This data-driven strategy can identify urinary metabolites associated with habitual exposure to specific foods. This trial has the UK registration number 4349 and was registered at isrtcn.org as CCT-NAPN-A13175.
BACKGROUND: An understanding of causal relations between diet and health is hindered by the lack of robust biological markers of food exposure. OBJECTIVE: We aimed to develop a data-driven procedure to discover urine biomarkers indicative of habitual exposure to different foods. DESIGN: The habitual diet of 68 participants was assessed by using 4 food-frequency questionnaires over 3 mo, and participants were assigned to different consumption-frequency classes for 58 dietary components. Flow infusion electrospray-ionization mass spectrometry followed by supervised multivariate data analysis was used to determine whether the chemical composition of urine was related to specific differences in the consumption levels of each food. RESULTS: Foods were eaten habitually in 1 of 5 basic patterns differing in range and distribution of consumption frequency. Overnight, 24-h, and fasting urine samples proved useful for biomarker lead discovery with habitual citrus exposure used as a paradigm. Exposure level discrimination robustness improved linearly as urine samples from low-frequency citrus consumers were compared with urine samples from participants reporting increasingly higher intakes. For all foods, distinctiveness and consumption-frequency range influenced the likelihood that differential dietary exposure could be detected. Model output statistics indicated foods for which biomarker lead discovery was feasible. Metabolites proposed previously as acute intake biomarkers of citrus (proline betaine), oily fish (methylhistidine), coffee (dihydrocaffeic acid derivatives), and tomato (phenolic metabolites) were also biomarkers of habitual exposure. A significance threshold in modeling output statistics was determined to guide the discovery of potential biomarkers for other foods. CONCLUSION: This data-driven strategy can identify urinary metabolites associated with habitual exposure to specific foods. This trial has the UK registration number 4349 and was registered at isrtcn.org as CCT-NAPN-A13175.
Authors: Pablo Hernández-Alonso; Christopher Papandreou; Mònica Bulló; Miguel Ruiz-Canela; Courtney Dennis; Amy Deik; Dong D Wang; Marta Guasch-Ferré; Edward Yu; Estefanía Toledo; Cristina Razquin; Dolores Corella; Ramon Estruch; Emilio Ros; Montserrat Fitó; Fernando Arós; Miquel Fiol; Lluís Serra-Majem; Liming Liang; Clary B Clish; Miguel A Martínez-González; Frank B Hu; Jordi Salas-Salvadó Journal: Mol Nutr Food Res Date: 2019-07-17 Impact factor: 5.914
Authors: Padma Maruvada; Johanna W Lampe; David S Wishart; Dinesh Barupal; Deirdra N Chester; Dylan Dodd; Yannick Djoumbou-Feunang; Pieter C Dorrestein; Lars O Dragsted; John Draper; Linda C Duffy; Johanna T Dwyer; Nancy J Emenaker; Oliver Fiehn; Robert E Gerszten; Frank B Hu; Robert W Karp; David M Klurfeld; Maren R Laughlin; A Roger Little; Christopher J Lynch; Steven C Moore; Holly L Nicastro; Diane M O'Brien; José M Ordovás; Stavroula K Osganian; Mary Playdon; Ross Prentice; Daniel Raftery; Nichole Reisdorph; Helen M Roche; Sharon A Ross; Shengmin Sang; Augustin Scalbert; Pothur R Srinivas; Steven H Zeisel Journal: Adv Nutr Date: 2020-03-01 Impact factor: 11.567
Authors: Cătălina Cuparencu; Åsmund Rinnan; Marta P Silvestre; Sally D Poppitt; Anne Raben; Lars O Dragsted Journal: Eur J Nutr Date: 2020-04-03 Impact factor: 5.614
Authors: Joseph A Rothwell; Francisco Madrid-Gambin; Mar Garcia-Aloy; Cristina Andres-Lacueva; Caomhan Logue; Alison M Gallagher; Carina Mack; Sabine E Kulling; Qian Gao; Giulia Praticò; Lars O Dragsted; Augustin Scalbert Journal: Genes Nutr Date: 2018-07-04 Impact factor: 5.523