Literature DB >> 23269729

Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus (MRSA) strains suitable in regions of high MRSA endemicity.

Jeong-Uk Kim1, Choong-Hwan Cha, Hae-Kyong An, Ho-Jun Lee, Mi-Na Kim.   

Abstract

A multiplex real-time PCR assay that simultaneously detects the mecA, staphylococcal cassette chromosome (SCCmec)-open reading frame X (orfX) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistant Staphylococcus aureus (MRSA) infections.

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Year:  2012        PMID： 23269729      PMCID： PMC3592057          DOI： 10.1128/JCM.02495-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  29 in total

1.  Does nasal cocolonization by methicillin-resistant coagulase-negative staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods?

Authors:  Karsten Becker; Isabelle Pagnier; Brigitte Schuhen; Frauke Wenzelburger; Alexander W Friedrich; Frank Kipp; Georg Peters; Christof von Eiff
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

2.  Classification of staphylococcal cassette chromosome mec (SCCmec): guidelines for reporting novel SCCmec elements.

Authors: 
Journal:  Antimicrob Agents Chemother       Date:  2009-08-31       Impact factor: 5.191

Review 3.  The emergence and evolution of methicillin-resistant Staphylococcus aureus.

Authors:  K Hiramatsu; L Cui; M Kuroda; T Ito
Journal:  Trends Microbiol       Date:  2001-10       Impact factor: 17.079

4.  Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs.

Authors:  Lance R Peterson; Oliver Liesenfeld; Christopher W Woods; Stephen D Allen; David Pombo; Parul A Patel; Maitry S Mehta; Bradly Nicholson; DeAnna Fuller; Andrew Onderdonk
Journal:  J Clin Microbiol       Date:  2010-03-24       Impact factor: 5.948

5.  Genotypic and phenotypic characterization of methicillin-susceptible Staphylococcus aureus isolates misidentified as methicillin-resistant Staphylococcus aureus by the BD GeneOhm MRSA assay.

Authors:  Paul D Stamper; Lisa Louie; Henry Wong; Andrew E Simor; Jason E Farley; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2011-02-09       Impact factor: 5.948

6.  Characterization of Staphylococcus aureus isolates with a partial or complete absence of staphylococcal cassette chromosome elements.

Authors:  Henry Wong; Lisa Louie; Reggie Y C Lo; Andrew E Simor
Journal:  J Clin Microbiol       Date:  2010-07-28       Impact factor: 5.948

7.  Molecular and epidemiological evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals.

Authors:  Pierre-Yves Donnio; Frédéric Février; Pablo Bifani; Marie Dehem; Christèle Kervégant; Nathalie Wilhelm; Anne-Lise Gautier-Lerestif; Nathalie Lafforgue; Michel Cormier; Alain Le Coustumier
Journal:  Antimicrob Agents Chemother       Date:  2007-08-20       Impact factor: 5.191

Review 8.  Molecular genetics of methicillin-resistant Staphylococcus aureus.

Authors:  Keiichi Hiramatsu; Yuki Katayama; Harumi Yuzawa; Teruyo Ito
Journal:  Int J Med Microbiol       Date:  2002-07       Impact factor: 3.473

9.  Multiplex real-time PCR for rapid Staphylococcal cassette chromosome mec typing.

Authors:  Liang Chen; José R Mediavilla; Duarte C Oliveira; Barbara M Willey; Herminia de Lencastre; Barry N Kreiswirth
Journal:  J Clin Microbiol       Date:  2009-09-02       Impact factor: 5.948

10.  Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study.

Authors:  Stephan Harbarth; Cristina Masuet-Aumatell; Jacques Schrenzel; Patrice Francois; Christophe Akakpo; Gesuele Renzi; Jerome Pugin; Bara Ricou; Didier Pittet
Journal:  Crit Care       Date:  2006-02       Impact factor: 9.097
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  8 in total

Review 1.  Wild-Type Gyrase A Genotype of Neisseria gonorrhoeae Predicts In Vitro Susceptibility to Ciprofloxacin: A Systematic Review of the Literature and Meta-Analysis.

Authors:  Lao-Tzu Allan-Blitz; Xiaoyan Wang; Jeffrey D Klausner
Journal:  Sex Transm Dis       Date:  2017-05       Impact factor: 2.830

2.  Evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test for detection of MRSA nasal colonization.

Authors:  W C Yam; Gilman K H Siu; P L Ho; T K Ng; T L Que; K T Yip; Cathie P K Fok; Jonathan H K Chen; Vincent C C Cheng; K Y Yuen
Journal:  J Clin Microbiol       Date:  2013-06-19       Impact factor: 5.948

3.  Prevalence of Mycoplasma genitalium and Azithromycin-resistant Infections Among Remnant Clinical Specimens, Los Angeles.

Authors:  Lao-Tzu Allan-Blitz; Elisa Mokany; Shelley Campeau; Rachel Wee; Chelsea Shannon; Jeffrey D Klausner
Journal:  Sex Transm Dis       Date:  2018-09       Impact factor: 2.830

4.  Accurate Detection of Methicillin-Resistant Staphylococcus aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR.

Authors:  Jun Luo; Junhua Li; Hang Yang; Junping Yu; Hongping Wei
Journal:  J Clin Microbiol       Date:  2017-07-19       Impact factor: 5.948

5.  Comparison between a chimeric lysin ClyH and other enzymes for extracting DNA to detect methicillin resistant Staphylococcus aureus by quantitative PCR.

Authors:  Yuanyuan Hu; Hang Yang; Jing Wang; Yun Zhang; Junping Yu; Hongping Wei
Journal:  World J Microbiol Biotechnol       Date:  2015-11-23       Impact factor: 3.312

Review 6.  Evolving Rapid Methicillin-resistant Staphylococcus aureus Detection: Cover All the Bases.

Authors:  Yamuna Devi Bakthavatchalam; Laura E B Nabarro; Balaji Veeraraghavan
Journal:  J Glob Infect Dis       Date:  2017 Jan-Mar

7.  Effect of delayed entry of blood culture bottles in BACTEC automated blood culture system in the context of laboratory consolidation.

Authors:  Vincent Deslandes; Darya Rafipour; Ivan Gorn; Elham Sabri; Nadia Sant; Marc Desjardins
Journal:  Sci Rep       Date:  2022-01-25       Impact factor: 4.379

8.  PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.

Authors:  Ying Liu; Jiang Zhang; Yinduo Ji
Journal:  Open Microbiol J       Date:  2016-04-14
  8 in total

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