BACKGROUND: Left ventricular noncompaction (LVNC) is a cardiomyopathy characterized by a prominent trabecular meshwork and deep intertrabecular recesses, and is thought to be due to an arrest of normal endomyocardial morphogenesis. However, the genes contributing to this process remain poorly understood. 14-3-3ε, encoded by YWHAE, is an adapter protein belonging to the 14-3-3 protein family which plays important roles in neuronal development and is involved in Miller-Dieker syndrome. We recently showed that mice lacking this gene develop LVNC. Therefore, we hypothesized that variants in YWHAE may contribute to the pathophysiology of LVNC in humans. METHODS AND RESULTS: In 77 Japanese patients with LVNC, including the probands of 29 families, mutation analysis of YWHAE by direct DNA sequencing identified 7 novel variants. One of them, c.-458G>T, in the YWHAE promoter, was identified in a familial patient with LVNC and hypoplasia of the corpus callosum. The -458G>T variant is located within a regulatory CCAAT/enhancer binding protein (C/EBP) response element of the YWHAE promoter, and it reduced promoter activity by approximately 50%. Increased binding of an inhibitory C/EBPβ isoform was implicated in decreasing YWHAE promoter activity. Interestingly, we had previously shown that C/EBPβ is a key regulator of YWHAE. CONCLUSIONS: These data suggest that the -458G>T YWHAE variant contributes to the abnormal myocardial morphogenesis characteristic of LVNC as well as abnormal brain development, and implicate YWHAE as a novel candidate gene in pediatric cardiomyopathies.
BACKGROUND: Left ventricular noncompaction (LVNC) is a cardiomyopathy characterized by a prominent trabecular meshwork and deep intertrabecular recesses, and is thought to be due to an arrest of normal endomyocardial morphogenesis. However, the genes contributing to this process remain poorly understood. 14-3-3ε, encoded by YWHAE, is an adapter protein belonging to the 14-3-3 protein family which plays important roles in neuronal development and is involved in Miller-Dieker syndrome. We recently showed that mice lacking this gene develop LVNC. Therefore, we hypothesized that variants in YWHAE may contribute to the pathophysiology of LVNC in humans. METHODS AND RESULTS: In 77 Japanese patients with LVNC, including the probands of 29 families, mutation analysis of YWHAE by direct DNA sequencing identified 7 novel variants. One of them, c.-458G>T, in the YWHAE promoter, was identified in a familial patient with LVNC and hypoplasia of the corpus callosum. The -458G>T variant is located within a regulatory CCAAT/enhancer binding protein (C/EBP) response element of the YWHAE promoter, and it reduced promoter activity by approximately 50%. Increased binding of an inhibitory C/EBPβ isoform was implicated in decreasing YWHAE promoter activity. Interestingly, we had previously shown that C/EBPβ is a key regulator of YWHAE. CONCLUSIONS: These data suggest that the -458G>T YWHAE variant contributes to the abnormal myocardial morphogenesis characteristic of LVNC as well as abnormal brain development, and implicate YWHAE as a novel candidate gene in pediatric cardiomyopathies.
Authors: Tao Tian; Jizheng Wang; Hu Wang; Kai Sun; Yilu Wang; Lei Jia; Yubao Zou; Rutai Hui; Xianliang Zhou; Lei Song Journal: Heart Vessels Date: 2014-04-02 Impact factor: 2.037
Authors: Adriana C Gittenberger-de Groot; Tamara Hoppenbrouwers; Lucile Miquerol; Yasuhiro Kosaka; Robert E Poelmann; Lambertus J Wisse; H Joseph Yost; Monique R M Jongbloed; Marco C Deruiter; Luca Brunelli Journal: Dev Dyn Date: 2016-09-18 Impact factor: 3.780
Authors: Kazuki Kodo; Sang-Ging Ong; Fereshteh Jahanbani; Vittavat Termglinchan; Keiichi Hirono; Kolsoum InanlooRahatloo; Antje D Ebert; Praveen Shukla; Oscar J Abilez; Jared M Churko; Ioannis Karakikes; Gwanghyun Jung; Fukiko Ichida; Sean M Wu; Michael P Snyder; Daniel Bernstein; Joseph C Wu Journal: Nat Cell Biol Date: 2016-09-19 Impact factor: 28.824