Literature DB >> 23265614

Agar barrier method for removal of yeast and other fungal contamination from Leishmania promastigotes culture.

Anil Kumar Gupta1, Shyam Narayan, Shubhankar Kumar Singh, Ajit Kumar Thakur, Pradeep Das.   

Abstract

The present study explains a novel method of Leishmania promastigotes culture decontamination. The method is based on motility of Leishmania promastigotes across agar barrier which facilitates decontamination from yeast and other non motile contamination. This is inexpensive, easy, rapid and reliable physical method and is able to save valuable isolates in culture.

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Year:  2012        PMID： 23265614      PMCID： PMC4001625          DOI： 10.1179/2047773212Y.0000000034

Source DB:  PubMed          Journal:  Pathog Glob Health        ISSN： 2047-7724            Impact factor:   2.894


  3 in total

1.  Removal of bacterial and yeast contamination from Leishmania promastigote cultures, by agar plating.

Authors:  M Muniaraj; A K Gupta; S Narayan; S Kumar; P K Sinha; K Kishore; P Das
Journal:  Ann Trop Med Parasitol       Date:  2005-09

2.  Mutant yeast on drugs.

Authors:  D J Lockhart
Journal:  Nat Med       Date:  1998-11       Impact factor: 53.440

3.  A rapid chromatographic method for elimination of fungal contamination in in vitro cultures of Leishmania spp.

Authors:  B M Okot-Kotber
Journal:  Parasitology       Date:  1985-08       Impact factor: 3.234
  3 in total
  1 in total

1.  Validation of Swab Sampling and SYBR Green-Based Real-Time PCR for the Diagnosis of Cutaneous Leishmaniasis in French Guiana.

Authors:  Romain Blaizot; Stéphane Simon; Marine Ginouves; Ghislaine Prévot; Denis Blanchet; Christophe Ravel; Pierre Couppie; Magalie Demar; Cécile Nabet
Journal:  J Clin Microbiol       Date:  2021-01-21       Impact factor: 5.948
  1 in total

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