PURPOSE: The purpose of this study was to evaluate the effect of whole bovine milk on Streptococcus mutans biofilms and on demineralization of enamel and root dentin. METHODS: S mutans UA159 biofilms were cultured on enamel and dentin bovine slabs of known surface hardness (SH) and exposed (8x/day) to 10% sucrose (positive control), whole bovine milk, and 4.5% lactose. Biofilm acidogenicity was estimated by medium pH (2x/day). After 4 days for dentin and 5 days for enamel, biofilms were collected to obtain biomass, viable bacteria, total protein, and intra- and extracellular polysaccharides. Final SH was measured on the slabs. Demineralization was calculated as percentage of SH loss (%SHL). Differences were determined by analysis of variance and Tukey's test (P<.05). RESULTS: Biofilms formed on both substrates under 10% sucrose showed higher acidogenicity, biomass, protein concentration, insoluble polysaccharides, and %SHL than those treated with milk or lactose (P<.05), which did not differ in any parameter (P>.05). Higher bacterial counts were obtained from biofilms treated with milk on enamel, but not on dentin. Dentin was significantly more demineralized by milk than enamel (P<.05). CONCLUSIONS: Although bovine milk appears to be less cariogenic than sucrose, this dairy product should not be considered caries-safe.
PURPOSE: The purpose of this study was to evaluate the effect of whole bovine milk on Streptococcus mutans biofilms and on demineralization of enamel and root dentin. METHODS:S mutans UA159 biofilms were cultured on enamel and dentin bovine slabs of known surface hardness (SH) and exposed (8x/day) to 10% sucrose (positive control), whole bovine milk, and 4.5% lactose. Biofilm acidogenicity was estimated by medium pH (2x/day). After 4 days for dentin and 5 days for enamel, biofilms were collected to obtain biomass, viable bacteria, total protein, and intra- and extracellular polysaccharides. Final SH was measured on the slabs. Demineralization was calculated as percentage of SH loss (%SHL). Differences were determined by analysis of variance and Tukey's test (P<.05). RESULTS: Biofilms formed on both substrates under 10% sucrose showed higher acidogenicity, biomass, protein concentration, insoluble polysaccharides, and %SHL than those treated with milk or lactose (P<.05), which did not differ in any parameter (P>.05). Higher bacterial counts were obtained from biofilms treated with milk on enamel, but not on dentin. Dentin was significantly more demineralized by milk than enamel (P<.05). CONCLUSIONS: Although bovine milk appears to be less cariogenic than sucrose, this dairy product should not be considered caries-safe.
Authors: Christopher A Schutt; Paul Neubauer; Boris Paskhover; Li Fang-Yong; Clarence T Sasaki Journal: Dysphagia Date: 2014-01-03 Impact factor: 3.438
Authors: Annette Carola Anderson; Michael Rothballer; Markus Jörg Altenburger; Johan Peter Woelber; Lamprini Karygianni; Kirstin Vach; Elmar Hellwig; Ali Al-Ahmad Journal: Appl Environ Microbiol Date: 2020-10-01 Impact factor: 4.792
Authors: Janelle Lee; Janice A Townsend; Tatyana Thompson; Thomas Garitty; Arpan De; Qingzhao Yu; Brian M Peters; Zezhang T Wen Journal: Caries Res Date: 2017-12-15 Impact factor: 4.056
Authors: Sohvi Lommi; Muhammed Manzoor; Elina Engberg; Nitin Agrawal; Timo A Lakka; Jukka Leinonen; Kaija-Leena Kolho; Heli Viljakainen Journal: Front Nutr Date: 2022-04-25