Literature DB >> 23264031

Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms (Bombyx mori).

Jianping Duan1, Hanfu Xu, Sanyuan Ma, Huizhen Guo, Feng Wang, Ping Zhao, Qingyou Xia.   

Abstract

Cre-mediated recombination is widely used to manipulate defined genes spatiotemporally in vivo. The present study evaluated the Cre/loxP system in Bombyx mori by establishing two transgenic lines. One line contained a Cre recombinase gene controlled by a sericin-1 gene (Ser1) promoter. The other line contained a loxP-Stop-loxP-DsRed cassette driven by the same Ser1 promoter. The precise deletion of the Stop fragment was found to be triggered by Cre-mediated site-specific excision, and led to the expression of DsRed fluorescence protein in the middle silk glands of all double-transgenic hybrids. This result was also confirmed by phenotypical analysis. Hence, the current study demonstrated the feasibility of Cre-mediated site-specific recombination in B. mori, and opened a new window for further refining genetic tools in silkworms.

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Year:  2012        PMID： 23264031     DOI： 10.1007/s11248-012-9677-0

Source DB:  PubMed          Journal:  Transgenic Res        ISSN： 0962-8819            Impact factor:   2.788


  41 in total

1.  Ends-out, or replacement, gene targeting in Drosophila.

Authors:  Wei J Gong; Kent G Golic
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-14       Impact factor: 11.205

2.  Identification of Ser2 proteins as major sericin components in the non-cocoon silk of Bombyx mori.

Authors:  Yoko Takasu; Tamako Hata; Keiro Uchino; Qiang Zhang
Journal:  Insect Biochem Mol Biol       Date:  2010-03-01       Impact factor: 4.714

3.  A targeted gene knockout in Drosophila.

Authors:  Y S Rong; K G Golic
Journal:  Genetics       Date:  2001-03       Impact factor: 4.562

4.  Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector.

Authors:  T Tamura; C Thibert; C Royer; T Kanda; E Abraham; M Kamba; N Komoto; J L Thomas; B Mauchamp; G Chavancy; P Shirk; M Fraser; J C Prudhomme; P Couble; T Toshiki; T Chantal; R Corinne; K Toshio; A Eappen; K Mari; K Natuo; T Jean-Luc; M Bernard; C Gérard; S Paul; F Malcolm; P Jean-Claude; C Pierre
Journal:  Nat Biotechnol       Date:  2000-01       Impact factor: 54.908

5.  Polyploidization of the silk gland of Bombyx mori.

Authors:  L P Gage
Journal:  J Mol Biol       Date:  1974-06-15       Impact factor: 5.469

6.  Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: comparison of the recombinase activity of the tamoxifen-inducible Cre-ER(T) and Cre-ER(T2) recombinases.

Authors:  A K Indra; X Warot; J Brocard; J M Bornert; J H Xiao; P Chambon; D Metzger
Journal:  Nucleic Acids Res       Date:  1999-11-15       Impact factor: 16.971

7.  Use of RNAi technology to confer enhanced resistance to BmNPV on transgenic silkworms.

Authors:  R Isobe; K Kojima; T Matsuyama; G X Quan; T Kanda; T Tamura; K Sahara; S I Asano; H Bando
Journal:  Arch Virol       Date:  2004-10       Impact factor: 2.574

8.  Targeted oncogene activation by site-specific recombination in transgenic mice.

Authors:  M Lakso; B Sauer; B Mosinger; E J Lee; R W Manning; S H Yu; K L Mulder; H Westphal
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

9.  Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori.

Authors:  Ken-ichiro Tatematsu; Isao Kobayashi; Keiro Uchino; Hideki Sezutsu; Tetsuya Iizuka; Naoyuki Yonemura; Toshiki Tamura
Journal:  Transgenic Res       Date:  2009-09-30       Impact factor: 2.788

10.  Analysis of tissue-specific region in sericin 1 gene promoter of Bombyx mori.

Authors:  Yan Liu; Lian Yu; Xiuyang Guo; Tingqing Guo; Shengpeng Wang; Changde Lu
Journal:  Biochem Biophys Res Commun       Date:  2006-02-06       Impact factor: 3.575
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  6 in total

Review 1.  Advanced technologies for genetically manipulating the silkworm Bombyx mori, a model Lepidopteran insect.

Authors:  Hanfu Xu; David A O'Brochta
Journal:  Proc Biol Sci       Date:  2015-07-07       Impact factor: 5.349

2.  Construction of transformed, cultured silkworm cells and transgenic silkworm using the site-specific integrase system from phage φC31.

Authors:  Yajuan Yin; Guangli Cao; Renyu Xue; Chengliang Gong
Journal:  Mol Biol Rep       Date:  2014-07-03       Impact factor: 2.316

3.  The C-terminus of DSX(F5) protein acts as a novel regulatory domain in Bombyx mori.

Authors:  Jianping Duan; Xianxin Meng; Sanyuan Ma; Feng Wang; Huozhen Guo; Liying Zhang; Ping Zhao; Yunchao Kan; Lunguang Yao; Qingyou Xia
Journal:  Transgenic Res       Date:  2016-03-15       Impact factor: 2.788

4.  Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system.

Authors:  Dingpei Long; Weijian Lu; Zhanzhang Hao; Zhonghuai Xiang; Aichun Zhao
Journal:  Transgenic Res       Date:  2016-06-22       Impact factor: 2.788

5.  An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori.

Authors:  Dingpei Long; Weijian Lu; Yuli Zhang; Lihui Bi; Zhonghuai Xiang; Aichun Zhao
Journal:  Sci Rep       Date:  2015-03-05       Impact factor: 4.379

6.  Aldehyde dehydrogenase-2 protects against myocardial infarction-related cardiac fibrosis through modulation of the Wnt/β-catenin signaling pathway.

Authors:  Xinjun Zhao; Yue Hua; Hongmei Chen; Haiyu Yang; Tao Zhang; Guiqiong Huang; Huijie Fan; Zhangbin Tan; Xiaofang Huang; Bin Liu; Yingchun Zhou
Journal:  Ther Clin Risk Manag       Date:  2015-09-11       Impact factor: 2.423
  6 in total

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