Literature DB >> 23263691

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast.

Sami Hocine1, Pascal Raymond, Daniel Zenklusen, Jeffrey A Chao, Robert H Singer.   

Abstract

Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

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Year:  2012        PMID： 23263691      PMCID： PMC3899799          DOI： 10.1038/nmeth.2305

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  20 in total

1.  Stochastic gene expression in a single cell.

Authors:  Michael B Elowitz; Arnold J Levine; Eric D Siggia; Peter S Swain
Journal:  Science       Date:  2002-08-16       Impact factor: 47.728

2.  Control of stochasticity in eukaryotic gene expression.

Authors:  Jonathan M Raser; Erin K O'Shea
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Review 3.  The highways and byways of mRNA decay.

Authors:  Nicole L Garneau; Jeffrey Wilusz; Carol J Wilusz
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4.  In vivo dynamics of RNA polymerase II transcription.

Authors:  Xavier Darzacq; Yaron Shav-Tal; Valeria de Turris; Yehuda Brody; Shailesh M Shenoy; Robert D Phair; Robert H Singer
Journal:  Nat Struct Mol Biol       Date:  2007-08-05       Impact factor: 15.369

Review 5.  Crossing the nuclear envelope: hierarchical regulation of nucleocytoplasmic transport.

Authors:  Laura J Terry; Eric B Shows; Susan R Wente
Journal:  Science       Date:  2007-11-30       Impact factor: 47.728

6.  Dissecting the regulatory circuitry of a eukaryotic genome.

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Review 7.  Nature, nurture, or chance: stochastic gene expression and its consequences.

Authors:  Arjun Raj; Alexander van Oudenaarden
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8.  Simultaneous transport of different localized mRNA species revealed by live-cell imaging.

Authors:  Susanne Lange; Yoshihiko Katayama; Maria Schmid; Ondrej Burkacky; Christoph Bräuchle; Don C Lamb; Ralf-Peter Jansen
Journal:  Traffic       Date:  2008-05-09       Impact factor: 6.215

Review 9.  RNA granules.

Authors:  Paul Anderson; Nancy Kedersha
Journal:  J Cell Biol       Date:  2006-03-06       Impact factor: 10.539

10.  A genomic integration method to visualize localization of endogenous mRNAs in living yeast.

Authors:  Liora Haim; Gadi Zipor; Stella Aronov; Jeffrey E Gerst
Journal:  Nat Methods       Date:  2007-04-08       Impact factor: 28.547
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  115 in total

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Authors:  Philip D Campbell; Jeffrey A Chao; Robert H Singer; Florence L Marlow
Journal:  Development       Date:  2015-03-10       Impact factor: 6.868

Review 2.  Using variability in gene expression as a tool for studying gene regulation.

Authors:  Olivia Padovan-Merhar; Arjun Raj
Journal:  Wiley Interdiscip Rev Syst Biol Med       Date:  2013-08-29

3.  Dual-colour imaging of RNAs using quencher- and fluorophore-binding aptamers.

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Review 4.  Single molecule studies of RNA polymerase II transcription in vitro.

Authors:  Abigail E Horn; James A Goodrich; Jennifer F Kugel
Journal:  Transcription       Date:  2014

Review 5.  Coming Together: RNAs and Proteins Assemble under the Single-Molecule Fluorescence Microscope.

Authors:  Ameya P Jalihal; Paul E Lund; Nils G Walter
Journal:  Cold Spring Harb Perspect Biol       Date:  2019-04-01       Impact factor: 10.005

Review 6.  Imaging the Life and Death of mRNAs in Single Cells.

Authors:  Jeffrey A Chao; Timothée Lionnet
Journal:  Cold Spring Harb Perspect Biol       Date:  2018-12-03       Impact factor: 10.005

7.  In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines.

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8.  mRNA quantification using single-molecule FISH in Drosophila embryos.

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Review 9.  Recent progress in single-molecule studies of mRNA localization in vivo.

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Journal:  RNA Biol       Date:  2018-11-14       Impact factor: 4.652

10.  The 4D nucleome project.

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Journal:  Nature       Date:  2017-09-13       Impact factor: 49.962
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