OBJECTIVE: The increased risk of cardiovascular diseases in postmenopausal women has been linked to the decrease in plasma estrogen levels. Preparation of conjugate equine estrogens (CEE) is one of the most routinely used hormone therapy in postmenopausal women. However, studies on the vascular effects of CEE are still sparse and the mechanism of action is not completely elucidated. In this context, we have determined the effects of CEE in the vascular oxidative stress observed in ovariectomyzed (OVX) spontaneously hypertensive rats (SHR). Mechanisms by which CEE interferes with redox-sensitive pathways and endothelial function were also determined. RESULTS: Aortas from OVX rats exhibited increased generation of reactive oxygen species (ROS), NADPH oxidase activity and reduced catalase protein expression, compared to aortas from sham SHR. Endothelium-intact aortic rings from OVX were hyperreactive to NE when compared to Sham aortas. This hyperreactivity was corrected by superoxide dismutase (SOD), catalase, and endothelium removal. Treatment of OVX-SHR with CEE reduced vascular ROS generation, NADPH oxidase activity, enhanced SOD and catalase expression and also corrected the NE-hyperreactivity in aortic rings from OVX-SHR. CONCLUSION: Our study indicates a potential benefit of CEE therapy through a mechanism that involves reduction in oxidative stress, improving endothelial function in OVX hypertensive rats.
OBJECTIVE: The increased risk of cardiovascular diseases in postmenopausal women has been linked to the decrease in plasma estrogen levels. Preparation of conjugate equine estrogens (CEE) is one of the most routinely used hormone therapy in postmenopausal women. However, studies on the vascular effects of CEE are still sparse and the mechanism of action is not completely elucidated. In this context, we have determined the effects of CEE in the vascular oxidative stress observed in ovariectomyzed (OVX) spontaneously hypertensiverats (SHR). Mechanisms by which CEE interferes with redox-sensitive pathways and endothelial function were also determined. RESULTS: Aortas from OVX rats exhibited increased generation of reactive oxygen species (ROS), NADPH oxidase activity and reduced catalase protein expression, compared to aortas from sham SHR. Endothelium-intact aortic rings from OVX were hyperreactive to NE when compared to Sham aortas. This hyperreactivity was corrected by superoxide dismutase (SOD), catalase, and endothelium removal. Treatment of OVX-SHR with CEE reduced vascular ROS generation, NADPH oxidase activity, enhanced SOD and catalase expression and also corrected the NE-hyperreactivity in aortic rings from OVX-SHR. CONCLUSION: Our study indicates a potential benefit of CEE therapy through a mechanism that involves reduction in oxidative stress, improving endothelial function in OVX hypertensiverats.
Authors: Tracey A Van Kempen; Ankita Narayan; Elizabeth M Waters; Jose Marques-Lopes; Costantino Iadecola; Michael J Glass; Virginia M Pickel; Teresa A Milner Journal: J Comp Neurol Date: 2015-12-23 Impact factor: 3.215
Authors: Carolina Falcão Ximenes; Samya Mere Lima Rodrigues; Priscila Lang Podratz; Eduardo Merlo; Julia Fernandez Puñal de Araújo; Lívia Carla Melo Rodrigues; Juliana Barbosa Coitinho; Dalton Valentim Vassallo; Jones Bernardes Graceli; Ivanita Stefanon Journal: Environ Sci Pollut Res Int Date: 2017-09-13 Impact factor: 4.223
Authors: Erick R G Claudio; Patrick W Endlich; Roger L Santos; Margareth R Moysés; Nazaré S Bissoli; Sônia A Gouvêa; Josiane F Silva; Virginia S Lemos; Glaucia R Abreu Journal: PLoS One Date: 2013-06-03 Impact factor: 3.240
Authors: Peter Slezak; Angelika Puzserova; Peter Balis; Natalia Sestakova; Miroslava Majzunova; Ima Dovinova; Michal Kluknavsky; Iveta Bernatova Journal: Biomed Res Int Date: 2014-03-05 Impact factor: 3.411